Antibody Fragment and Affibody ImmunoPET Imaging Agents: Radiolabelling Strategies and Applications.
Antibody Fragment and Affibody ImmunoPET Imaging Agents: Radiolabelling Strategies and Applications.
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DOI：
10.1002/cmdc.201800624
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发表时间：
2018-12-06
期刊：
影响因子：
3.4
通讯作者：
Miller PW
中科院分区：
文献类型：
--
作者：
Fu R;Carroll L;Yahioglu G;Aboagye EO;Miller PW
长期以来，抗体一直被认为是将诊断医学放射性核素、造影剂和光学探针携带到病变组织进行成像的有效载体。免疫PET领域结合了正电子发射断层扫描(PET)成像和抗体的使用，以改进疾病的诊断、分期和监测。抗体工程和PET放射化学的最新进展导致了基于一系列抗体片段和亲和体的新一波实验性免疫PET显像剂的出现。与全抗体相比，工程亲和蛋白和抗体片段，如微型体、双胞胎、单链可变区片段(ScFv)和纳米体要小得多，但除了具有更理想的成像药代动力学外，还保留了全抗体的基本特异性和亲和力。在这里，重点介绍了抗体片段和相关的亲和体分子的PET放射性标记策略的最新进展，以及过表达的抗原相关肿瘤和免疫细胞的主要PET成像应用。
Antibodies have long been recognised as potent vectors for carrying diagnostic medical radionuclides, contrast agents and optical probes to diseased tissue for imaging. The area of ImmunoPET combines the use of positron emission tomography (PET) imaging with antibodies to improve the diagnosis, staging and monitoring of diseases. Recent developments in antibody engineering and PET radiochemistry have led to a new wave of experimental ImmunoPET imaging agents that are based on a range of antibody fragments and affibodies. In contrast to full antibodies, engineered affibody proteins and antibody fragments such as minibodies, diabodies, single‐chain variable region fragments (scFvs), and nanobodies are much smaller but retain the essential specificities and affinities of full antibodies in addition to more desirable pharmacokinetics for imaging. Herein, recent key developments in the PET radiolabelling strategies of antibody fragments and related affibody molecules are highlighted, along with the main PET imaging applications of overexpressed antigen‐associated tumours and immune cells.
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影响因子：
3.1
作者：
Dearling JL;Voss SD;Dunning P;Snay E;Fahey F;Smith SV;Huston JS;Meares CF;Treves ST;Packard AB
通讯作者：
Packard AB
影响因子：
4.7
作者：
Agarwal, Paresh;Bertozzi, Carolyn R.
通讯作者：
Bertozzi, Carolyn R.
影响因子：
3.6
作者：
Freise AC;Wu AM
通讯作者：
Wu AM
影响因子：
3.2
作者：
Carroll, Laurence;Evans, Helen L.;Spivey, Alan C.
通讯作者：
Spivey, Alan C.
影响因子：
4.7
作者：
Chakravarty, Rubel;Goel, Shreya;Valdovinos, Hector F.;Hernandez, Reinier;Hong, Hao;Nickles, Robert J.;Cai, Weibo
通讯作者：
Cai, Weibo