In vivo evaluation of injectable thermosensitive polymer with time-dependent LCST.
In vivo evaluation of injectable thermosensitive polymer with time-dependent LCST.
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DOI：
10.1002/jbm.a.32179
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发表时间：
2009-09-15
影响因子：
4.9
通讯作者：
Vernon, Brent L.
中科院分区：
文献类型：
--
作者：
Henderson, Eric;Lee, Bae Hoon;Cui, Zhanwu;McLemore, Ryan;Brandon, Tedd A.;Vernon, Brent L.
本研究的重点是研究由NIPAAm、二甲基-γ-丁内酯（DMBL）和丙烯酸（AAc）组成的共聚物体系的生物相容性、随时间变化的LCST和生物可蚀性。大鼠皮下注射25 wt%聚（NIPAAm-co-DMBL-co-AAc）溶液。在预定的时间，动物被杀死，聚合物植入物被回收，通过1H-NMR进行表征。此外，收集与聚合物接触的组织切片并进行组织学处理。通过计算组织-植入物界面上存在的成纤维细胞和白细胞的数量来评估植入物的生物相容性。从体内植入物获得的LCST数据与体外研究结果一致。4天后，植入物的质量下降，表明扩散速度加快，植入物肿胀增加，1H-NMR测量证实了水解降解。在植入后30天，共聚物植入物-组织界面的细胞存在恢复到正常组织的水平，这表明伤口愈合反应正常。
The focus of this study was to examine the biocompatibility, time-dependent LCST, and bioerodable properties of a copolymer system composed of NIPAAm, Dimethyl-γ-Butyrolactone (DMBL), and Acrylic Acid (AAc). Sprague Dawley rats were subcutaneously injected with 25 wt% solutions of Poly(NIPAAm-co-DMBL-co-AAc). At predetermined times, animals were sacrificed and polymer implants were recovered for characterization via 1H-NMR. In addition, polymer-contacting tissue sections were harvested and processed for histology. The biocompatibility of the implants was assessed by counting the number of fibroblasts and leukocytes present at the tissue-implant interface. The LCST data obtained from the in vivo implants was shown to agree with that of in vitro findings. Implant mass was shown to decrease after 4 days, indicating accelerated diffusion rates with increased implant swelling, hydrolytic degradation was confirmed with 1H-NMR measurements. The cellular presence at the copolymer implant-tissue interface was shown to return to that of normal tissue 30 days post-implantation, which suggests a normal wound healing response.
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