Tissue-specific venous expression of the EPH family receptor EphB1 in the skin vasculature.
Tissue-specific venous expression of the EPH family receptor EphB1 in the skin vasculature.
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DOI：
10.1002/dvdy.23985
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发表时间：
2013-08
影响因子：
2.5
通讯作者：
Mukouyama, Yoh-suke
中科院分区：
文献类型：
--
作者：
Li, Wenling;Mukouyama, Yoh-suke
大动脉和静脉在发育早期就形成了。鉴别动脉和静脉内皮细胞的分子工具提高了我们对动脉-静脉分化和分支形态发生的理解。与动脉分化相比，由于静脉内皮细胞缺乏明确的分子标记，人们对控制静脉发育的因素知之甚少。在这里，我们报告了针对EphB1 （EphB类受体）的抗体，使得在胚胎和成人皮肤血管模型中建立可靠的静脉身份全安装免疫组织化学分析成为可能，其分辨率比以前更高。从胚胎发育到成年，EphB1的表达仅限于整个静脉血管，而先前建立的静脉标记物EphB4也可在淋巴血管中检测到。EphB1的静脉限制性表达是在初级毛细血管丛的血管重构发生后建立的。然而，与在皮肤中的静脉特异性表达相比，EphB1并不局限于卵黄囊、躯干和肺部的静脉血管。这些研究以组织特异性和时间依赖性的方式介绍了EphB1作为一种新的静脉限制性标志物。
The major arteries and veins are formed early during development. The molecular tools to identify arterial and venous endothelial cells improve our understanding of arterial-venous differentiation and branching morphogenesis. Compared to arterial differentiation, relatively little is known about what controls venous development, due to a lack of definitive molecular markers for venous endothelial cells. Here we report that the antibody against EphB1, an EphB class receptor, makes it possible to establish a reliable whole-mount immunohistochemical analysis of venous identity with greater resolution than previously possible in embryonic and adult skin vasculature models. EphB1 expression is restricted to the entire venous vasculature throughout embryonic development to adulthood, whereas the previously established venous marker EphB4 is also detectable in lymphatic vasculature. This venous-restricted expression of EphB1 is established after the vascular remodeling of the primary capillary plexus has occurred. Compared to its venous-specific expression in the skin, however, EphB1 is not restricted to the venous vasculature in yolk sac, trunk and lung. These studies introduce EphB1 as a new venous-restricted marker in a tissue-specific and time-dependent manner.
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影响因子：
64.5
作者：
Mukouyama, Y;Shin, D;Anderson, DJ
通讯作者：
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