Molecular approaches for improving desiccation tolerance: insights from the brine shrimp Artemia franciscana.
Molecular approaches for improving desiccation tolerance: insights from the brine shrimp Artemia franciscana.
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DOI：
10.1007/s00425-015-2281-9
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发表时间：
2015-08
期刊：
影响因子：
4.3
通讯作者：
Menze MA
中科院分区：
文献类型：
--
作者：
Hand SC;Menze MA
栖息在水生和陆地生态系统中的生物经常面临因多种原因而导致的水分流失问题——暴露于高盐度、蒸发性水分流失以及由于细胞外液冻结而限制细胞内水分。季节性干燥可能会变得严重，并导致产生耐受的繁殖体并在生命周期的各个阶段进入缺水状态。丰年虾（Artemia franciscana）的原肠胚期胚胎就是这种情况。对干燥的生理和生化反应是生存的核心，并且是多方面的。本综述将评估源自 A. franciscana 的多种晚期胚胎发生丰富 (LEA) 蛋白与非还原糖海藻糖对预防生物组织多个层面的干燥损伤的影响。通过使用上述保护剂，结合代谢预处理和快速细胞干燥，可以提高水分胁迫期间干燥敏感细胞的存活率。然而，在室温下获得干燥状态的细胞的长期稳定性尚未实现，需要在物理化学和生物学方面继续努力。
Organisms inhabiting both aquatic and terrestrial ecosystems frequently are confronted with the problem of water loss for multiple reasons – exposure to hypersalinity, evaporative water loss, and restriction of intracellular water due to freezing of extracellular fluids. Seasonal desiccation can become severe and lead to the production of tolerant propagules and entry into the state of anhydrobiosis at various stages of the life cycle. Such is the case for gastrula-stage embryos of the brine shrimp, Artemia franciscana. Physiological and biochemical responses to desiccation are central for survival and are multifaceted. This review will evaluate the impact of multiple Late Embryogenesis Abundant (LEA) proteins originating from A. franciscana, together with the non-reducing sugar trehalose, on prevention of desiccation damage at multiple levels of biological organization. Survivorship of desiccation-sensitive cells during water stress can be improved by use of the above protective agents, coupled to metabolic preconditioning and rapid cell drying. However, obtaining long-term stability of cells in the dried state at room temperature has not been accomplished and will require continued efforts on both the physicochemical and biological fronts.
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影响因子：
4.4
作者：
Clark, Melody S;Thorne, Michael As;Purac, Jelena;Grubor-Lajsic, Gordana;Kube, Michael;Reinhardt, Richard;Worland, M Roger
通讯作者：
Worland, M Roger
影响因子：
2.6
作者：
Boswell, Leaf C.;Hand, Steven C.
通讯作者：
Hand, Steven C.
影响因子：
4.4
作者：
Foerster, Frank;Liang, Chuanguang;Dandekar, Thomas
通讯作者：
Dandekar, Thomas
影响因子：
2.2
作者：
Hand, Steven C.;Menze, Michael A.;Borcar, Apu;Patil, Yuvraj;Covi, Joseph A.;Reynolds, Julie A.;Toner, Mehmet
通讯作者：
Toner, Mehmet
影响因子：
4.1
作者：
Goyal, K;Walton, LJ;Tunnacliffe, A
通讯作者：
Tunnacliffe, A