A nuclear import pathway exploited by pathogenic noncoding RNAs.
A nuclear import pathway exploited by pathogenic noncoding RNAs.
复制标题
DOI：
10.1093/plcell/koac210
复制
发表时间：
2022-09-27
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
真核细胞中细胞内RNA运输的流行观点是，在细胞核中转录的RNA要么留在细胞核中，要么穿过核膜，进入细胞质发挥功能。然而，新出现的证据表明，许多功能性RNA的移动方向相反，从细胞质到核。RNA核输入的机制尚未得到很好的阐明。类病毒是感染植物的单链环状非编码RNA。使用本氏烟草（Nicotiana benthamiana）、番茄（Solanum lycopersicum）和核复制类病毒作为模型，我们表明细胞IMPORTIN ALPHA-4（IMPA-4）可能参与类病毒RNA核输入，经验上支持基于Importin的细胞途径参与RNA核输入。我们还证实了与IMPA-4和类病毒结合的细胞蛋白（类病毒RNA结合蛋白1 [VIRP 1]）的参与。此外，核复制类病毒中保守的C环是核输入的关键信号。破坏C环会损害VIRP 1结合、类病毒核积累和感染性。此外，C环存在于依赖于VIRP 1进行核输入的亚病毒卫星非编码RNA中。这些结果推进了我们对亚病毒RNA感染和RNA核输入调控的理解。RNA C环基序是VIRP 1识别的一个关键信号，用于通过基于IMPORTIN ALPHA-4的细胞途径向核输入致病性非编码RNA（即核复制类病毒和可能的病毒卫星RNA）。
The prevailing view of intracellular RNA trafficking in eukaryotic cells is that RNAs transcribed in the nucleus either stay in the nucleus or cross the nuclear envelope, entering the cytoplasm for function. However, emerging evidence illustrates that numerous functional RNAs move in the reverse direction, from the cytoplasm to the nucleus. The mechanism underlying RNA nuclear import has not been well elucidated. Viroids are single-stranded circular noncoding RNAs that infect plants. Using Nicotiana benthamiana, tomato (Solanum lycopersicum), and nuclear-replicating viroids as a model, we showed that cellular IMPORTIN ALPHA-4 (IMPa-4) is likely involved in viroid RNA nuclear import, empirically supporting the involvement of Importin-based cellular pathway in RNA nuclear import. We also confirmed the involvement of a cellular protein (viroid RNA-binding protein 1 [VIRP1]) that binds both IMPa-4 and viroids. Moreover, a conserved C-loop in nuclear-replicating viroids serves as a key signal for nuclear import. Disrupting C-loop impairs VIRP1 binding, viroid nuclear accumulation, and infectivity. Further, C-loop exists in a subviral satellite noncoding RNA that relies on VIRP1 for nuclear import. These results advance our understanding of subviral RNA infection and the regulation of RNA nuclear import. RNA C-loop motif is a key signal recognized by the VIRP1 for the nuclear import of pathogenic noncoding RNAs (i.e. nuclear-replicating viroids and possibly a viral satellite RNA), via the IMPORTIN ALPHA-4-based cellular pathway.
登录
查看更多内容
核中植物病毒RNA基因组中的鉴定。
影响因子：
3.7
作者：
Gao R;Liu P;Wong SM
通讯作者：
Wong SM
DOI：
10.1073/pnas.1316579110
发表时间：
2013-12-24
影响因子：
11.1
作者：
Kramer, Emily B.;Hopper, Anita K.
通讯作者：
Hopper, Anita K.
影响因子：
5.6
作者：
Gast, FU;Kempe, D;Sanger, HL
通讯作者：
Sanger, HL
影响因子：
56.9
作者：
Frey, Steffen;Richter, Ralf P.;Goerlich, Dirk
通讯作者：
Goerlich, Dirk
影响因子：
3.7
作者：
Giguère T;Adkar-Purushothama CR;Perreault JP
通讯作者：
Perreault JP