Comprehensive secondary structure elucidation of four genera of the family Pospiviroidae.
Comprehensive secondary structure elucidation of four genera of the family Pospiviroidae.
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DOI：
10.1371/journal.pone.0098655
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Perreault JP
中科院分区：
文献类型：
--
作者：
Giguère T;Adkar-Purushothama CR;Perreault JP
类病毒是感染植物的小的环状单链RNA分子。由于它们是非编码的，它们的结构在它们的生命周期中起着关键作用。到目前为止，很少有人花精力阐明类病毒的结构，由于实验的困难和耗时的性质所涉及的方法。近年来，高通量引物延伸分析技术（SHAPE）被应用于检测Avsunviroidae家族中在叶绿体中复制并具有核酶活性的成员。在本工作中，对属于痘病毒科四个不同属的12种类病毒进行了研究，其成员的特征是存在中央保守区（CCR）并在宿主细胞核中复制。鉴于五个不同的类病毒科的结构已被报道，概述了所有属的不同结构特征和开始的手动分类的不同类病毒的基础上，其结构特征在这里。
Viroids are small, circular, single stranded RNA molecules that infect plants. Since they are non-coding, their structures play a critical role in their life cycles. To date, little effort has been spend on elucidating viroid structures in solution due to both the experimental difficulties and the time-consuming nature of the methodologies implicated. Recently, the technique of high-throughput selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) was adapted for the probing of the members of family Avsunviroidae, all of whom replicate in the chloroplast and demonstrate ribozyme activity. In the present work, twelve viroid species belonging to four different genera of the family Pospiviroidae, whose members are characterized by the presence of a central conserved region (CCR) and who replicate in nucleus of the host, were probed. Given that the structures of five distinct viroid species from the family Pospiviroidae have been previously reported, an overview of the different structural characteristics for all genera and the beginning of a manual classification of the different viroids based on their structural features are presented here.
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影响因子：
4.5
作者：
Karabiber, Fethullah;McGinnis, Jennifer L.;Weeks, Kevin M.
通讯作者：
Weeks, Kevin M.
DOI：
10.1073/pnas.1219988110
发表时间：
2013-04-02
影响因子：
11.1
作者：
Hajdin, Christine E.;Bellaousov, Stanislav;Weeks, Kevin M.
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Weeks, Kevin M.
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作者：
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影响因子：
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作者：
HASELOFF, J;SYMONS, RH
通讯作者：
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