Molecular cloning of Indian hedgehog gene and its expression in golden pompano Trachinotus ovatus (Linnaeus 1758) larvae at different water temperatures
Molecular cloning of Indian hedgehog gene and its expression in golden pompano Trachinotus ovatus (Linnaeus 1758) larvae at different water temperatures
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印度刺猬基因的分子克隆及其在卵形金鲳鲹(Linnaeus 1758)幼虫中不同水温下的表达
DOI：
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yu G.
中科院分区：
文献类型：
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作者：
Ma Z;Fu M. Hu J. Yang R. Wei C. Qin J.G.;Yu G.
克隆并分析了金色海棠幼体中的印度刺猬(IHH)基因。在仔鱼个体发育的前18天检测IHH的表达，然后在孵化后18天(DPH)检测IHH在鱼组织中的表达。随后，在12DPH和18DPH下，比较了IHH对23、26和29℃水温的响应。该基因的全长为1,484bp，开放阅读框为1,314 bp。该基因编码437个氨基酸，计算相对分子质量为48.175 kDa，理论等电点为6.22。孵化后，IHH的表达随着鱼龄的增加而增加，并在3DPH时达到高峰。在4~12DPH之间波动后，IHH的表达在18DPH时达到第二高峰。在18DPH时，IHH在鱼肠组织中的表达最高，其次是在胃中。水温显著影响IHH的表达。在12DPH时，23oC时IHH的表达显著高于26oC和29oC时的表达。在18DPH时，26℃和29℃时IHH的表达显著高于23℃时的表达。本研究检测了IHH基因在金腹鱼发育早期的表达，IHH在仔鱼中的时间依赖性表达对于了解仔鱼成骨的个体发育具有重要意义。
The Indian hedgehog (Ihh) gene in golden pompano larvae was cloned and analyzed. The expression of Ihh during larval fish ontogeny in the first 18 days was examined, and then the expression of Ihh in fish tissues was evaluated on 18 days post hatching (DPH). Subsequently, the response of Ihh to water temperatures of 23, 26, and 29 C was compared on 12 DPH and 18 DPH. The cDNA sequence length of the golden pompano Ihh gene is 1,484 bp with an open reading frame of 1,314 bp. The Ihh gene encodes 437 amino acids and has a calculated molecular weight of 48.175 kDa and a theoretical isoelectric point of 6.22. After hatching, the expression of Ihh increased with fish age and peaked at 3 DPH. After fluctuations between 4 and 12 DPH, the expression of Ihh reached the second peak at 18 DPH. The highest expression of Ihh in tissues occurred in the fish intestine, followed in the stomach on 18 DPH. Water temperature significantly affected Ihh expression. On 12 DPH, the expression of Ihh at 23oC was significantly higher than that at 26 and 29 oC. In contrast, the expression of Ihh at 26 and 29 C was significantly higher than that at 23 oC on 18 DPH. This study detected the gene expression of Ihh at the early stage of golden pompano development and the time-dependent expression of Ihh in fish larvae is important to understand the ontogeny of bone formation in fish larvae.
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