Re-evaluation of the near infrared spectra of mitochondrial cytochrome c oxidase: Implications for non invasive in vivo monitoring of tissues.
Re-evaluation of the near infrared spectra of mitochondrial cytochrome c oxidase: Implications for non invasive in vivo monitoring of tissues.
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DOI：
10.1016/j.bbabio.2014.08.005
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发表时间：
2014-11
影响因子：
4.3
通讯作者：
Cooper, Chris E.
中科院分区：
文献类型：
--
作者：
Mason, Maria G.;Nicholls, Peter;Cooper, Chris E.
我们重新测定了不同细胞色素c氧化酶氧化还原中心的近红外（NIR）光谱特征（650-980 nm），并将它们分离到它们的组成物种。我们确认氧化酶近红外光谱在700 ~ 980 nm之间的主要贡献者是铜CuA，其在牛肉心脏酶中在835 nm处具有最大值。655 nm波段表征了完全氧化的血红素a3/CuB双核中心；当一个或多个电子被添加到双核中心或当后者被配体修饰时，它被漂白。由此产生的“扰动”双核中心也具有先前未报道的715 - 920nm宽波段。某些稳定配体（甲酸酯和CO）与不稳定氧反应化合物P和F的近红外光谱相似，表明后者可能在电子上与稳定配体相似。血红素a的氧化还原对835 nm最大值和715 nm波段均无贡献。我们的结果证实了近红外光谱在体内监测细胞色素氧化酶活性的CuA中心的能力，尽管注意到在血红素a3/CuB双核中心存在扰动的情况下，精确定量解释存在一些困难。对细胞色素氧化酶的近红外光谱进行了解卷积。在700 ~ 980 nm之间的主要特征为CuA发色团。血红素和铁中心没有显著的贡献。血红素a3/CuB双核中心在715 ~ 920 nm之间有一个新的特征。体内氧中间体P和F浓度的变化可以测量。
We re-determined the near infrared (NIR) spectral signatures (650–980 nm) of the different cytochrome c oxidase redox centres, in the process separating them into their component species. We confirm that the primary contributor to the oxidase NIR spectrum between 700 and 980 nm is cupric CuA, which in the beef heart enzyme has a maximum at 835 nm. The 655 nm band characterises the fully oxidised haem a3/CuB binuclear centre; it is bleached either when one or more electrons are added to the binuclear centre or when the latter is modified by ligands. The resulting ‘perturbed’ binuclear centre is also characterised by a previously unreported broad 715–920 nm band. The NIR spectra of certain stable liganded species (formate and CO), and the unstable oxygen reaction compounds P and F, are similar, suggesting that the latter may resemble the stable species electronically. Oxidoreduction of haem a makes no contribution either to the 835 nm maximum or the 715 nm band. Our results confirm the ability of NIRS to monitor the CuA centre of cytochrome oxidase activity in vivo, although noting some difficulties in precise quantitative interpretations in the presence of perturbations of the haem a3/CuB binuclear centre. The NIR spectrum of cytochrome oxidase was deconvoluted into its component species. The dominant feature between 700 and 980 nm was confirmed as the CuA chromophore. There was no significant contribution from the haem a iron centre. A new feature between 715 and 920 nm was assigned to the haem a3/CuB binuclear centre. Changes in concentrations of oxygen intermediates P and F may be measurable in vivo.
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影响因子：
4.1
作者：
HENNING, W;VO, L;HILL, BC
通讯作者：
HILL, BC
影响因子：
2.9
作者：
Jasaitis, A;Backgren, C;Wikström, M
通讯作者：
Wikström, M
DOI：
10.1085/jgp.46.5.905
发表时间：
1963-05
期刊：
The Journal of general physiology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1098/rspb.1925.0039
发表时间：
1925-08-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-CONTAINING PAPERS OF A BIOLOGICAL CHARACTER
影响因子：
--
作者：
Keilin, D
通讯作者：
Keilin, D
DOI：
10.1098/rstb.1997.0048
发表时间：
1997-06-29
影响因子：
6.3
作者：
Cooper, CE;Springett, R
通讯作者：
Springett, R