The R2R3 MYB transcription factors FOUR LIPS and MYB88 regulate female reproductive development.
The R2R3 MYB transcription factors FOUR LIPS and MYB88 regulate female reproductive development.
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DOI：
10.1093/jxb/ers209
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发表时间：
2012-09
影响因子：
6.9
通讯作者：
Lamb RS
中科院分区：
文献类型：
--
作者：
Makkena S;Lee E;Sack FD;Lamb RS
配子形成是有性生殖生物生命周期的重要步骤。在开花植物中，孢子母细胞减数分裂后形成单倍体孢子。这些孢子经过有丝分裂后发育成含有配子的雄性和雌性配子体。在雌性中，大孢子母细胞经历减数分裂，形成四个大孢子，其中一个是功能性的，三个是退化的。然后大孢子经历三个有丝分裂周期，从而产生具有八个核的胚囊。胚囊经历细胞化形成成熟的七细胞雌配子体。减数分裂的进入和进展对于大孢子发生和随后的大配子发生至关重要，但对该过程的控制尚不清楚。四唇 (FLP) 及其旁系同源物 MYB88（编码 R2R3 MYB 转录因子）已被广泛研究，因为它们通过直接调节细胞周期基因的表达来限制气孔发育的终末分裂。这里证明FLP和MYB88也调节女性生殖。 FLP 和 MYB88 均在胚珠发育过程中表达，它们的缺失会显着增加胎盘产生的胚珠数量。尽管存在过多的胚珠，但单突变体和双突变体由于雌性生育力降低而表现出结籽率减少。不育至少部分是由有缺陷的减数分裂进入和进展造成的。因此，FLP 和 MYB88 是进入大孢子发生的重要调节因子，并且可能通过细胞周期基因的调节发挥作用。
Gamete formation is an important step in the life cycle of sexually reproducing organisms. In flowering plants, haploid spores are formed after the meiotic division of spore mother cells. These spores develop into male and female gametophytes containing gametes after undergoing mitotic divisions. In the female, the megaspore mother cell undergoes meiosis forming four megaspores, of which one is functional and three degenerate. The megaspore then undergoes three mitotic cycles thus generating an embryo sac with eight nuclei. The embryo sac undergoes cellularization to form the mature seven-celled female gametophyte. Entry into and progression through meiosis is essential for megasporogenesis and subsequent megagametogenesis, but control of this process is not well understood. FOUR LIPS (FLP) and its paralogue MYB88, encoding R2R3 MYB transcription factors, have been extensively studied for their role in limiting the terminal division in stomatal development by direct regulation of the expression of cell cycle genes. Here it is demonstrated that FLP and MYB88 also regulate female reproduction. Both FLP and MYB88 are expressed during ovule development and their loss significantly increases the number of ovules produced by the placenta. Despite the presence of excess ovules, single and double mutants exhibit reduced seed set due to reduced female fertility. The sterility results at least in part from defective meiotic entry and progression. Therefore, FLP and MYB88 are important regulators of entry into megasporogenesis, and probably act via the regulation of cell cycle genes.
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DOI：
10.1101/sqb.1997.062.01.007
发表时间：
1997-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
Moore, JM;Calzada, JPV;Grossniklaus, U
通讯作者：
Grossniklaus, U
影响因子：
9.2
作者：
Hunt, Lee;Gray, Julie E.
通讯作者：
Gray, Julie E.
影响因子：
7.2
作者：
Colombo, Monica;Masiero, Simona;Colombo, Lucia
通讯作者：
Colombo, Lucia
影响因子：
4.5
作者：
d'Erfurth I;Cromer L;Jolivet S;Girard C;Horlow C;Sun Y;To JP;Berchowitz LE;Copenhaver GP;Mercier R
通讯作者：
Mercier R
影响因子：
4.6
作者：
Gremski, Kristina;Ditta, Gary;Yanofsky, Martin F.
通讯作者：
Yanofsky, Martin F.