Identification of Long Noncoding RNA Expression Profiles in HPV-Negative Cervical Cancer
Identification of Long Noncoding RNA Expression Profiles in HPV-Negative Cervical Cancer
复制标题
HPV 阴性宫颈癌中长非编码 RNA 表达谱的鉴定
DOI：
10.1159/000510030
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Shuzhong Yao
中科院分区：
文献类型：
--
作者：
Shiyin Ooi;Yu;ong Liao;Pan Liu;Ganlin Xu;Tianyu Liu;Shuzhong Yao
目的：与HPV阳性病例相比，HPV阴性宫颈癌（CC）通常更具侵袭性，导致生存结局较差。然而，关于HPV阴性CC的研究很少，相关的分子机制尚不清楚。本研究旨在探索长链非编码RNA（lncRNA）在宫颈癌组织中的表达谱，并筛选出可能作为HPV阴性宫颈癌潜在生物标志物的肿瘤相关lncRNA。研究方法：利用生物信息学方法构建lncRNA的表达谱，进行Gene Ontology和KEGG分析，绘制lncRNA-mRNA共表达网络。通过qRT-PCR检测前5个标记的肿瘤相关lncRNA的表达水平。采用Cell Counting Kit-8和transwell法研究LINC 00115对CC生长和转移的影响。结果：与正常宫颈（NC）相比，2，052个lncRNA在HPV阴性CC中差异表达。结果表明，LINC 00115在HPV阴性CC细胞中的表达显著上调，并能促进HPV阴性CC细胞的增殖、迁移和侵袭。结论：LINC 00115可能是HPV阴性CC的潜在生物标志物。
Aim: HPV-negative cervical cancer (CC) usually appears more aggressive and causes poorer survival outcomes compared to HPV-positive cases. However, the research in regard to HPV-negative CC is rare, and the related molecular mechanism underlying remains unclear. We intended to explore the expression profiles of long noncoding RNAs (lncRNAs) and identify the tumor-associated lncRNAs which might be used as the potential biomarker for HPV-negative CC. Methods: Bioinformatics analyses were utilized to construct the expression profiles of lncRNAs, Gene Ontology, and KEGG analyses and draw the lncRNA-mRNA co-expression network in HPV-negative CC. The expression levels of the top 5 marked-up tumor-associated lncRNAs were detected by qRT-PCR. The effect of LINC00115 on CC growth and metastasis was studied by Cell Counting Kit-8 and transwell assays. Results: In comparison to normal cervix (NC), 2,052 lncRNAs were differentially expressed in HPV-negative CC. It demonstrated that LINC00115 was significantly upregulated in HPV-negative CC cells compared to NC, and it could promote proliferation, migration, and invasion of HPV-negative CC cells. Conclusion: LINC00115 might be a potential biomarker for HPV-negative CC.
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DOI：
10.1016/0140-6736(90)92693-c
发表时间：
1990-05
期刊：
The Lancet
影响因子：
--
作者：
G. Riou;M. Fãvre;D. Jeannel;J. Bourhis;V. Doussal;G. Orth
通讯作者：
G. Riou;M. Fãvre;D. Jeannel;J. Bourhis;V. Doussal;G. Orth
DOI：
10.1186/s13046-016-0445-5
发表时间：
2016-10-28
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
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作者：
Liu TY;Chen J;Shang CL;Shen HW;Huang JM;Liang YC;Wang W;Zhao YH;Liu D;Shu M;Guo LY;Hu Z;Yao SZ
通讯作者：
Yao SZ
影响因子：
51.1
作者：
de Sanjose, Silvia;Quint, Wim G. V.;Xavier Bosch, F.
通讯作者：
Xavier Bosch, F.
DOI：
10.1097/pgp.0000000000000132
发表时间：
2015-03-01
影响因子：
2.4
作者：
Casey, Shauna;Harley, Ian;McCluggage, W. Glenn
通讯作者：
McCluggage, W. Glenn
DOI：
10.1111/1471-0528.13071
发表时间：
2015-01-01
影响因子：
5.8
作者：
Rodriguez-Carunchio, L.;Soveral, I.;del Pino, M.
通讯作者：
del Pino, M.