Distinct septin heteropolymers co-exist during multicellular development in the filamentous fungus Aspergillus nidulans.
Distinct septin heteropolymers co-exist during multicellular development in the filamentous fungus Aspergillus nidulans.
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DOI：
10.1371/journal.pone.0092819
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Momany M
中科院分区：
文献类型：
--
作者：
Hernández-Rodríguez Y;Masuo S;Johnson D;Orlando R;Smith A;Couto-Rodriguez M;Momany M
隔膜蛋白是细胞骨架的重要组成部分，在真核生物中高度保守，并在胞质分裂、图案形成和许多发育过程中发挥重要作用。隔膜形成杂聚物，其组装成更高级的结构，包括环、细丝和纱布。与肌动蛋白丝和微管相反，septins组装的分子机制还不清楚。在这里，我们报告说，在丝状真菌构巢曲霉，四个核心septins形成杂聚复合物。AspE是单细胞酵母中缺乏的第五种隔蛋白，仅与一种核心隔蛋白相互作用，并且仅在多细胞生长期间。AspE是三种核心隔蛋白正确定位所必需的，并且需要核心隔蛋白的相同子集用于其自身独特的皮质定位。ΔaspE突变体缺乏发育特异性隔蛋白高级结构，并且在低温和渗透胁迫下显示出孢子产生减少和生长缓慢。我们的研究结果表明，至少有两个不同的septin杂聚体群体共存于A。虽然AspE不是任一杂聚物的亚基，但它是多细胞发育中发现的隔蛋白高阶结构组装所需的。
Septins are important components of the cytoskeleton that are highly conserved in eukaryotes and play major roles in cytokinesis, patterning, and many developmental processes. Septins form heteropolymers which assemble into higher-order structures including rings, filaments, and gauzes. In contrast to actin filaments and microtubules, the molecular mechanism by which septins assemble is not well-understood. Here, we report that in the filamentous fungus Aspergillus nidulans, four core septins form heteropolymeric complexes. AspE, a fifth septin lacking in unicellular yeasts, interacts with only one of the core septins, and only during multicellular growth. AspE is required for proper localization of three of the core septins, and requires this same subset of core septins for its own unique cortical localization. The ΔaspE mutant lacks developmentally-specific septin higher-order structures and shows reduced spore production and slow growth with low temperatures and osmotic stress. Our results show that at least two distinct septin heteropolymer populations co-exist in A. nidulans, and that while AspE is not a subunit of either heteropolymer, it is required for assembly of septin higher-order structures found in multicellular development.
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影响因子：
3.4
作者：
Pan F;Malmberg RL;Momany M
通讯作者：
Momany M
影响因子：
3.7
作者：
Berepiki A;Read ND
通讯作者：
Read ND
纯化的酵母菌的聚合：Septin功能可能不需要有组织的丝阵列。
影响因子：
7.8
作者：
Frazier, J A;Wong, M L;Longtine, M S;Pringle, J R;Mann, M;Mitchison, T J;Field, C
通讯作者：
Field, C
影响因子：
19
作者：
Oh Y;Bi E
通讯作者：
Bi E
影响因子：
7.8
作者：
Garcia, Galo, III;Bertin, Aurelie;Nogales, Eva
通讯作者：
Nogales, Eva