Analysis of septins across kingdoms reveals orthology and new motifs.
Analysis of septins across kingdoms reveals orthology and new motifs.
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DOI：
10.1186/1471-2148-7-103
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发表时间：
2007-07-01
影响因子：
3.4
通讯作者：
Momany M
中科院分区：
文献类型：
--
作者：
Pan F;Malmberg RL;Momany M
Septins是在酵母菌中首次发现的细胞骨架GT3蛋白，它们在酵母菌中组织隔膜并将核分裂与细胞分裂联系起来。最近在动物中发现了septins，它们在从肌动蛋白和微管组织到胚胎形成的过程中非常重要，并且septins的缺陷与人类疾病有关。以前的研究表明，许多动物septins属于独立的进化群体，混淆了跨王国的比较。本研究从真菌、微孢子虫和动物中鉴定了162个septins，并分析了它们的系统发育关系。有五组septins与王国之间的正误支持。第1组（包括S.酿酒酵母Cdc10p和人Sept 9）和组2（其包括S.酿酒酵母Cdc3p和人Sept 7）含有来自真菌和动物的序列。第3组（包括S.酿酒酵母Cdc11p）和第4组（包括S.酿酒酵母Cdc12p）含有来自真菌和微孢子虫的序列。第5组（包括构巢曲霉AspE）含有来自丝状真菌的序列。我们建议修改命名的基础上，这些系统发育关系。比较序列比对显示septin衍生物的已知G1，G3和G4 GT3基序，4个新的基序从2至12个氨基酸长和6个保守的单一氨基酸的位置。这些新的基序之一是septin特异性的，几个是组特异性的。我们的研究为这个重要的蛋白质家族提供了进化历史，并为跨王国的septin直系同源物的比较提供了框架和一致的命名法。
Septins are cytoskeletal GTPase proteins first discovered in the fungus Saccharomyces cerevisiae where they organize the septum and link nuclear division with cell division. More recently septins have been found in animals where they are important in processes ranging from actin and microtubule organization to embryonic patterning and where defects in septins have been implicated in human disease. Previous studies suggested that many animal septins fell into independent evolutionary groups, confounding cross-kingdom comparison. In the current work, we identified 162 septins from fungi, microsporidia and animals and analyzed their phylogenetic relationships. There was support for five groups of septins with orthology between kingdoms. Group 1 (which includes S. cerevisiae Cdc10p and human Sept9) and Group 2 (which includes S. cerevisiae Cdc3p and human Sept7) contain sequences from fungi and animals. Group 3 (which includes S. cerevisiae Cdc11p) and Group 4 (which includes S. cerevisiae Cdc12p) contain sequences from fungi and microsporidia. Group 5 (which includes Aspergillus nidulans AspE) contains sequences from filamentous fungi. We suggest a modified nomenclature based on these phylogenetic relationships. Comparative sequence alignments revealed septin derivatives of already known G1, G3 and G4 GTPase motifs, four new motifs from two to twelve amino acids long and six conserved single amino acid positions. One of these new motifs is septin-specific and several are group specific. Our studies provide an evolutionary history for this important family of proteins and a framework and consistent nomenclature for comparison of septin orthologs across kingdoms.
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