Laboratory Diagnosis of SARS-CoV-2 Pneumonia.
Laboratory Diagnosis of SARS-CoV-2 Pneumonia.
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DOI：
10.3390/diagnostics11071270
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发表时间：
2021-07-15
期刊：
影响因子：
--
通讯作者：
Sordillo EM
中科院分区：
文献类型：
--
作者：
Gitman MR;Shaban MV;Paniz-Mondolfi AE;Sordillo EM
冠状病毒病-2019年的出现和迅速扩散在过去一年里给全球卫生基础设施和卫生经济模式带来了前所未有的压力。时间紧迫的议程--识别人类中的病毒并向公众提供疫苗--构成了一种努力，目的是在病毒呈指数增长时使其传播的统计曲线变得平坦。在这一努力的前沿，迫切需要制定快速和准确的诊断策略。在过去的一年里，这些问题以各种形式出现--每种形式都有优势和劣势。到目前为止，它们分为三类：(1)从呼吸道患者样本中分离和复制病毒RNA的方法(核酸扩增测试；NAAT)；(2)检测病毒蛋白存在的方法(快速抗原测试；老鼠)；以及基于血清学的检查，确定全血和血清中的病毒抗体。后者在检测早期和晚期感染中已知流行率的免疫球蛋白方面有所不同。在这篇综述中，我们描述了迄今为止开发的检测措施的类别，分析了从呼吸道不同区域收集患者样本的有效性，并介绍了使病原体识别更容易和更容易为公众所接受的新兴技术。
The emergence and rapid proliferation of Coronavirus Disease-2019, throughout the past year, has put an unprecedented strain on the global schema of health infrastructure and health economy. The time-sensitive agenda of identifying the virus in humans and delivering a vaccine to the public constituted an effort to flatten the statistical curve of viral spread as it grew exponentially. At the forefront of this effort was an exigency of developing rapid and accurate diagnostic strategies. These have emerged in various forms over the past year—each with strengths and weaknesses. To date, they fall into three categories: (1) those isolating and replicating viral RNA in patient samples from the respiratory tract (Nucleic Acid Amplification Tests; NAATs), (2) those detecting the presence of viral proteins (Rapid Antigen Tests; RATs) and serology-based exams identifying antibodies to the virus in whole blood and serum. The latter vary in their detection of immunoglobulins of known prevalence in early-stage and late-stage infection. With this review, we delineate the categories of testing measures developed to date, analyze the efficacy of collecting patient specimens from diverse regions of the respiratory tract, and present the up and coming technologies which have made pathogen identification easier and more accessible to the public.
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影响因子：
5.9
作者：
Bosch, Berend Jan;Smits, Saskia L.;Haagmans, Bart L.
通讯作者：
Haagmans, Bart L.
DOI：
10.1016/j.dsx.2020.04.020
发表时间：
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9.4
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