Yielding Behavior in Injectable Hydrogels from Telechelic Proteins.
Yielding Behavior in Injectable Hydrogels from Telechelic Proteins.
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DOI：
10.1021/ma101434a
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发表时间：
2010-11-09
期刊：
影响因子：
5.5
通讯作者：
Tirrell DA
中科院分区：
文献类型：
--
作者：
Olsen BD;Kornfield JA;Tirrell DA
可注射水凝胶在微创组织工程和药物输送过程中显示出巨大的应用前景。一种由大肠杆菌中表达的重组遥爪蛋白开发而成的新型可注射水凝胶材料，在大菌株中表现出三个数量级的剪切稀化。大幅度振荡剪切表明剪切稀化是由于凝胶主体内的屈服造成的，并且流变响应和流动曲线与屈服的剪切带机制一致。急剧的屈服转变和大幅度的表观剪切稀化使得凝胶只需轻轻的手压即可通过窄规格针头注射。注射后，由于物理网络连接的快速重组，凝胶在几秒钟内恢复到完全弹性强度，从而形成自支撑结构。剪切稀化和恢复行为在很大程度上独立于中间嵌段长度，使得基因工程可用于控制凝胶的平衡模量，而不损失特征屈服行为。剪切带机制将流动过程中的变形局部化到凝胶的狭窄区域，从而使 95% 以上的种子细胞能够在注射过程中存活下来。
Injectable hydrogels show substantial promise for use in minimally invasive tissue engineering and drug delivery procedures. A new injectable hydrogel material, developed from recombinant telechelic proteins expressed in E. coli, demonstrates shear thinning by three orders of magnitude at large strains. Large amplitude oscillatory shear illustrates that shear thinning is due to yielding within the bulk of the gel, and the rheological response and flow profiles are consistent with a shear-banding mechanism for yielding. The sharp yielding transition and large magnitude of the apparent shear thinning allow gels to be injected through narrow gauge needles with only gentle hand pressure. After injection the gels reset to full elastic strength in seconds due to rapid reformation of the physical network junctions, allowing self-supporting structures to be formed. The shear thinning and recovery behavior is largely independent of the midblock length, enabling genetic engineering to be used to control the equilibrium modulus of the gel without loss of the characteristic yielding behavior. The shear-banding mechanism localizes deformation during flow into narrow regions of the gels, allowing more than 95% of seeded cells to survive the injection process.
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