Genome evolution and phylogenomic analysis of Candidatus Kinetoplastibacterium, the betaproteobacterial endosymbionts of Strigomonas and Angomonas.
Genome evolution and phylogenomic analysis of Candidatus Kinetoplastibacterium, the betaproteobacterial endosymbionts of Strigomonas and Angomonas.
复制标题
DOI：
10.1093/gbe/evt012
复制
发表时间：
2013
影响因子：
3.3
通讯作者：
Buck GA
中科院分区：
文献类型：
--
作者：
Alves JM;Serrano MG;Maia da Silva F;Voegtly LJ;Matveyev AV;Teixeira MM;Camargo EP;Buck GA
人们早就知道，来自Angomonas和Strigomonas属的感染昆虫的锥虫鞭毛虫携带补充宿主代谢的细菌内共生体（Trypanosomatid proteobacterial endosynbiont）（Trypanosomatid kinetoplastibacterium或TPE）。基于先前对来自其他谱系的其他细菌内共生体基因组的分析，已经表征了在这些细菌与真核宿主相关联的持续时间内的基因组进化的常规路径。在这项工作中，我们测序和分析五个TPE的基因组，进行代谢重建，做了广泛的基因组分析与所有可用的β-变形菌，并比较TPE与其最近的β-变形菌亲属。我们还确定了一些管家和中央代谢基因，似乎已经经历了积极的选择。我们的基因组结构分析显示，尽管存在数百万年的分歧，但五种TPE之间存在总的同线性，并且这种谱系遵循在不同祖先的其他内共生体中观察到的基因组进化的共同路径。正如以前所建议的细胞生物学和生物化学实验，钙。动塑性杆菌属优先保持那些对于它们的宿主所需的化合物的生物合成所必需的基因。我们还表明，代谢和信息基因与主机的合作是过度的基因之间显示，是积极的选择。最后，我们的基因组分析表明，虽然在Alcaligenaceae家庭的β-变形菌，这些内共生体的最接近的亲戚是不是在属博德特氏菌如先前报道的，但更可能在泰勒属。
It has been long known that insect-infecting trypanosomatid flagellates from the genera Angomonas and Strigomonas harbor bacterial endosymbionts (Candidatus Kinetoplastibacterium or TPE [trypanosomatid proteobacterial endosymbiont]) that supplement the host metabolism. Based on previous analyses of other bacterial endosymbiont genomes from other lineages, a stereotypical path of genome evolution in such bacteria over the duration of their association with the eukaryotic host has been characterized. In this work, we sequence and analyze the genomes of five TPEs, perform their metabolic reconstruction, do an extensive phylogenomic analyses with all available Betaproteobacteria, and compare the TPEs with their nearest betaproteobacterial relatives. We also identify a number of housekeeping and central metabolism genes that seem to have undergone positive selection. Our genome structure analyses show total synteny among the five TPEs despite millions of years of divergence, and that this lineage follows the common path of genome evolution observed in other endosymbionts of diverse ancestries. As previously suggested by cell biology and biochemistry experiments, Ca. Kinetoplastibacterium spp. preferentially maintain those genes necessary for the biosynthesis of compounds needed by their hosts. We have also shown that metabolic and informational genes related to the cooperation with the host are overrepresented amongst genes shown to be under positive selection. Finally, our phylogenomic analysis shows that, while being in the Alcaligenaceae family of Betaproteobacteria, the closest relatives of these endosymbionts are not in the genus Bordetella as previously reported, but more likely in the Taylorella genus.
登录
查看更多内容
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
3.4
作者：
Anisimova M;Bielawski J;Dunn K;Yang Z
通讯作者：
Yang Z
DOI：
10.1016/0006-291x(92)91838-h
发表时间：
1992-02-14
影响因子：
3.1
作者：
CAMPBELL, DA
通讯作者：
CAMPBELL, DA
影响因子：
56.9
作者：
CHANG, KP;TRAGER, W
通讯作者：
TRAGER, W
影响因子：
4.1
作者：
Kueck, Patrick;Meusemann, Karen
通讯作者：
Meusemann, Karen