Genome-wide association mapping for seedling and adult plant resistance to stripe rust in synthetic hexaploid wheat.
Genome-wide association mapping for seedling and adult plant resistance to stripe rust in synthetic hexaploid wheat.
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整个基因组的关联映射，用于幼苗和成年植物对六倍小麦的抗条纹生锈的抗性。
DOI：
10.1371/journal.pone.0105593
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ogbonnaya FC
中科院分区：
文献类型：
--
作者：
Zegeye H;Rasheed A;Makdis F;Badebo A;Ogbonnaya FC
利用相关野生和驯化物种的遗传多样性对提高小麦产量做出了重大贡献。人工合成六倍体小麦（SHWs）表现出对生物和非生物胁迫的抗性和/或耐受性的天然遗传变异。条锈菌（Puccinia striiformis f.）小麦赤霉病是世界性的小麦重要病害。为了研究小麦条锈病抗性基因座，我们利用小麦9 K SNP iSelect基因芯片对181个小麦条锈病抗性基因座进行了全基因组关联分析（GWAS）。评估了SHW在幼苗和成株阶段对Pst流行小种的反应，后者于2011年在埃塞俄比亚的两个地点进行了重复田间试验。在Meraro和Arsi长袍，约28%的SHWs在苗期表现出免疫，而在成株期分别有56%和83%的SHWs对Pst具有抗性。在1BS、2AS、2BL、3BL、3DL、5A、5 BL、6DS和7A等9个基因组区域共发现27个与苗期抗瘟性相关的SNPs，在18个基因组区域发现38个与成株期抗瘟性相关的SNPs。六个基因组区域通常检测到在两个位置使用混合线性模型校正人口结构，亲缘关系和调整错误发现率（FDR）。染色体区域1AS，3DL，6DS和7AL上的位点似乎是新的QTL，我们的研究结果证实，再合成小麦涉及其祖先物种是一个丰富的来源，新的条（黄）锈病抗性，可能是有用的选择SHWs和纳入不同的黄锈病（YR）抗性位点到当地适应的小麦品种。
Use of genetic diversity from related wild and domesticated species has made a significant contribution to improving wheat productivity. Synthetic hexaploid wheats (SHWs) exhibit natural genetic variation for resistance and/or tolerance to biotic and abiotic stresses. Stripe rust caused by (Puccinia striiformis f. sp. tritici; Pst), is an important disease of wheat worldwide. To characterise loci conferring resistance to stripe rust in SHWs, we conducted a genome-wide association study (GWAS) with a panel of 181 SHWs using the wheat 9K SNP iSelect array. The SHWs were evaluated for their response to the prevailing races of Pst at the seedling and adult plant stages, the latter in replicated field trials at two sites in Ethiopia in 2011. About 28% of the SHWs exhibited immunity at the seedling stage while 56% and 83% were resistant to Pst at the adult plant stage at Meraro and Arsi Robe, respectively. A total of 27 SNPs in nine genomic regions (1BS, 2AS, 2BL, 3BL, 3DL, 5A, 5BL, 6DS and 7A) were linked with resistance to Pst at the seedling stage, while 38 SNPs on 18 genomic regions were associated with resistance at the adult plant stage. Six genomic regions were commonly detected at both locations using a mixed linear model corrected for population structure, kinship relatedness and adjusted for false discovery rate (FDR). The loci on chromosome regions 1AS, 3DL, 6DS and 7AL appeared to be novel QTL; our results confirm that resynthesized wheat involving its progenitor species is a rich source of new stripe (yellow) rust resistance that may be useful in choosing SHWs and incorporating diverse yellow rust (YR) resistance loci into locally adapted wheat cultivars.
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影响因子：
3.3
作者：
Breseghello, F;Sorrells, ME
通讯作者：
Sorrells, ME
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2.3
作者：
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影响因子：
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作者：
Guo, Jun;Bai, Pengfei;Kang, Zhensheng
通讯作者：
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