A plant reovirus hijacks endoplasmic reticulum-associated degradation machinery to promote efficient viral transmission by its planthopper vector under high temperature conditions.
A plant reovirus hijacks endoplasmic reticulum-associated degradation machinery to promote efficient viral transmission by its planthopper vector under high temperature conditions.
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植物呼肠孤病毒劫持内质网相关降解机制，以促进其飞虱载体在高温条件下有效传播病毒。
DOI：
10.1371/journal.ppat.1009347
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发表时间：
2021-03
期刊：
影响因子：
6.7
通讯作者：
Wei T
中科院分区：
文献类型：
--
作者：
Yu X;Jia D;Wang Z;Li G;Chen M;Liang Q;Zhou Y;Liu H;Xiao M;Li S;Chen Q;Chen H;Wei T
在田间，许多虫媒作物病毒病更适合在高温地区维持和传播，但其机制仍知之甚少。稻飞虱传播的水稻呼肠孤病毒（南方水稻黑条矮缩病毒，SRBSDV）在我国南方和越南北方地区的流行，主要集中在高温地区。在此，我们报道了内质网相关降解（ERAD）机制的两个因子，热休克蛋白DnaJB 11和ER膜蛋白BAP 31，被病毒感染激活，介导S. furcifera高温。SRBSDV在S.白背飞虱随温度升高而增加。我们观察到高温（35°C）有利于由SRBSDV的非结构蛋白P7-1形成的含病毒管状结构的组装，这有助于通过S. furcifera。DnaJB 11和BAP 31都能竞争性地与SRBSDV的P7-1蛋白结合，但DnaJB 11促进P7- 1蛋白在内质网膜上的组装，而BAP 31则抑制P7-1蛋白在内质网膜上的组装。此外，DnaJB 11与P7-1的结合亲和力强于BAP 31与P7-1的结合亲和力。我们还发现BAP 31通过它们的直接相互作用负调控DnaJB 11的表达。高温可显著上调DnaJB 11的表达，但抑制BAP 31的表达，从而强烈促进丰富的P7-1小管的组装。综上所述，我们发现ERAD机制中一种新的温度依赖性蛋白质质量控制途径已经进化为DnaJB 11的强激活，以有利于P7-1小管组装，从而支持SRBSDV在高温下的有效传播。因此，我们推断，ERAD机制已搭便车由昆虫传播的作物病毒，以提高其在热带气候中的传播。
In the field, many insect-borne crop viral diseases are more suitable for maintenance and spread in hot-temperature areas, but the mechanism remains poorly understood. The epidemic of a planthopper (Sogatella furcifera)-transmitted rice reovirus (southern rice black-streaked dwarf virus, SRBSDV) is geographically restricted to southern China and northern Vietnam with year-round hot temperatures. Here, we reported that two factors of endoplasmic reticulum-associated degradation (ERAD) machinery, the heat shock protein DnaJB11 and ER membrane protein BAP31, were activated by viral infection to mediate the adaptation of S. furcifera to high temperatures. Infection and transmission efficiencies of SRBSDV by S. furcifera increased with the elevated temperatures. We observed that high temperature (35°C) was beneficial for the assembly of virus-containing tubular structures formed by nonstructural protein P7-1 of SRBSDV, which facilitates efficient viral transmission by S. furcifera. Both DnaJB11 and BAP31 competed to directly bind to the tubule protein P7-1 of SRBSDV; however, DnaJB11 promoted whereas BAP31 inhibited P7-1 tubule assembly at the ER membrane. Furthermore, the binding affinity of DnaJB11 with P7-1 was stronger than that of BAP31 with P7-1. We also revealed that BAP31 negatively regulated DnaJB11 expression through their direct interaction. High temperatures could significantly upregulate DnaJB11 expression but inhibit BAP31 expression, thereby strongly facilitating the assembly of abundant P7-1 tubules. Taken together, we showed that a new temperature-dependent protein quality control pathway in the ERAD machinery has evolved for strong activation of DnaJB11 for benefiting P7-1 tubules assembly to support efficient transmission of SRBSDV in high temperatures. We thus deduced that ERAD machinery has been hitchhiked by insect-borne crop viruses to enhance their transmission in tropical climates.
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影响因子：
4.6
作者：
Anfoka G;Moshe A;Fridman L;Amrani L;Rotem O;Kolot M;Zeidan M;Czosnek H;Gorovits R
通讯作者：
Gorovits R
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影响因子：
6.7
作者：
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影响因子：
5.4
作者：
Jia, Dongsheng;Mao, Qianzhuo;Wei, Taiyun
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Wei, Taiyun
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影响因子：
6.7
作者：
Huo Y;Liu W;Zhang F;Chen X;Li L;Liu Q;Zhou Y;Wei T;Fang R;Wang X
通讯作者：
Wang X
影响因子：
2.9
作者：
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通讯作者：
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