Transovarial transmission of a plant virus is mediated by vitellogenin of its insect vector.
Transovarial transmission of a plant virus is mediated by vitellogenin of its insect vector.
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植物病毒的跨卵巢传播是由其昆虫载体的卵黄蛋白原介导的
DOI：
10.1371/journal.ppat.1003949
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发表时间：
2014-03
期刊：
影响因子：
6.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Huo Y;Liu W;Zhang F;Chen X;Li L;Liu Q;Zhou Y;Wei T;Fang R;Wang X
大多数植物病毒是由半翅目昆虫传播的。一些病毒通常可以通过卵子从母本传播给后代，但这种经卵子传播的机制尚不清楚。水稻条纹病毒是一种细小病毒，主要由褐飞虱传播，也通过卵传播给后代。在这里，我们以RSV-稻飞虱系统为模型，研究了纹枯病菌卵黄蛋白原(VG)在病毒进入卵子传播过程中的中心作用。我们的数据表明，VG在体内和体外都能与PC3结合，并在细菌中共定位。RSV丝状核糖核蛋白颗粒(RNPs)在VG表达前仅聚集在终丝和花梗区域，在VG表达之前不存在于生殖器中，RSV RNPs和VG在VG表达后共存。免疫电子显微镜(IEM)观察也表明，这两种蛋白共存于哺乳细胞中。RNA干扰抑制VG的表达可抑制RSV对卵巢的侵袭。综上所述，这些数据表明RSV RNPs可能通过与VG结合，通过内吞作用进入细菌的哺育细胞。最后，病毒通过营养脐带进入卵母细胞，使用与VG运输相同的途径。我们的结果表明，纹状钩体的VG在RSV的经卵传播中发挥了关键作用，并说明了病毒如何利用昆虫媒介中现有的经卵转运系统来达到自己的目的。
Most plant viruses are transmitted by hemipteroid insects. Some viruses can be transmitted from female parent to offspring usually through eggs, but the mechanism of this transovarial transmission remains unclear. Rice stripe virus (RSV), a Tenuivirus, transmitted mainly by the small brown planthopper (Laodelphax striatellus), is also spread to the offspring through the eggs. Here, we used the RSV–planthopper system as a model to investigate the mechanism of transovarial transmission and demonstrated the central role of vitellogenin (Vg) of L. striatellus in the process of virus transmission into the eggs. Our data showed Vg can bind to pc3 in vivo and in vitro and colocalize in the germarium. RSV filamentous ribonucleoprotein particles (RNPs) only accumulated in the terminal filaments and pedicel areas prior to Vg expression and was not present in the germarium until Vg was expressed, where RSV RNPs and Vg had colocalized. Observations by immunoelectron microscopy (IEM) also indicated that these two proteins colocalized in nurse cells. Knockdown of Vg expression due to RNA interference resulted in inhibition of the invasion of ovarioles by RSV. Together, the data obtained indicated that RSV RNPs may enter the nurse cell of the germarium via endocytosis through binding with Vg. Finally, the virus enters the oocytes through nutritive cords, using the same route as for Vg transport. Our results show that the Vg of L. striatellus played a critical role in transovarial transmission of RSV and shows how viruses can use existing transovarial transportation systems in insect vectors for their own purposes.
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