Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis.
Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis.
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放射性肠炎肠道微生物组成和脂质代谢特征的改变
DOI：
10.3389/fcimb.2020.541178
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发表时间：
2020
影响因子：
5.7
通讯作者：
Ding Y
中科院分区：
文献类型：
--
作者：
Li Y;Yan H;Zhang Y;Li Q;Yu L;Li Q;Liu C;Xie Y;Chen K;Ye F;Wang K;Chen L;Ding Y
放射性肠炎是癌症病人接受放射线治疗时常见的并发症。虽然研究已经显示了该病在临床、病理等层面的变化，但其局部微生物组学和代谢组学的动态特征迄今尚不清楚。我们的目的是研究肠道微生态系统的多组学特征，确定RE活动期间微生物组和脂质代谢产物之间的功能相关性。通过单次大剂量照射，建立RE小鼠模型。进行高通量16 S rDNA测序和全局脂质组学分析，以检查肠道微生态系统中的微生物和脂质组学特征的变化。采用斯皮尔曼相关分析确定细菌与代谢产物之间的功能相关性。收集临床样本以验证上述观察结果。在RE活动期间，小鼠肠道炎症通过典型的体征、症状、影像学表现和病理学证据得到证实。16 S数据集显示，局部照射显著改变了肠道微生物组成，导致拟杆菌门与厚壁菌门的比例降低。脂质组学分析表明肠上皮屏障中的显著脂质组学特征变化，确定甘油磷脂代谢与RE进展相关性最高。斯皮尔曼相关分析表明，在RE中，5种细菌-代谢物对表现出最显著的功能相关性，包括Alistipes-PC（36：0 e）、Bacteroides-DG（18：0/20：4）、Dubosiella-PC（35：2）、Eggerthellaceae-PC（35：6）和土方ichia-Shigella-TG（18：2/18：2/20：4）。这些观察结果在人体标本中得到了部分证实。我们的研究提供了一个全面的描述微生物群失调和脂质代谢紊乱的RE，提出了策略，以改变局部微生态系统，以减轻辐射损伤和维持稳态。
Radiation enteritis (RE) is a common complication in cancer patients receiving radiotherapy. Although studies have shown the changes of this disease at clinical, pathological and other levels, the dynamic characteristics of local microbiome and metabolomics are hitherto unknown. We aimed to examine the multi-omics features of the gut microecosystem, determining the functional correlation between microbiome and lipid metabolites during RE activity. By delivering single high-dose irradiation, a RE mouse model was established. High-throughput 16S rDNA sequencing and global lipidomics analysis were performed to examine microbial and lipidomic profile changes in the gut microecosystem. Spearman correlation analysis was used to determine the functional correlation between bacteria and metabolites. Clinical samples were collected to validate the above observations. During RE activity, the intestinal inflammation of the mice was confirmed by typical signs, symptoms, imaging findings and pathological evidences. 16S datasets revealed that localized irradiation dramatically altered the gut microbial composition, resulting in a decrease ratio of Bacteroidetes to Firmicutes. Lipidomics analysis indicated the remarkable lipidomic profile changes in enteric epithelial barrier, determining that glycerophospholipids metabolism was correlated to RE progression with the highest relevance. Spearman correlation analysis identified that five bacteria-metabolite pairs showed the most significant functional correlation in RE, including Alistipes-PC(36:0e), Bacteroides-DG(18:0/20:4), Dubosiella-PC(35:2), Eggerthellaceae-PC(35:6), and Escherichia-Shigella-TG(18:2/18:2/20:4). These observations were partly confirmed in human specimens. Our study provided a comprehensive description of microbiota dysbiosis and lipid metabolic disorders in RE, suggesting strategies to change local microecosystem to relieve radiation injury and maintain homeostasis.
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影响因子：
5.6
作者：
Banna GL;Torino F;Marletta F;Santagati M;Salemi R;Cannarozzo E;Falzone L;Ferraù F;Libra M
通讯作者：
Libra M
影响因子：
2.9
作者：
Han, XL;Yang, JY;Gross, RW
通讯作者：
Gross, RW
DOI：
10.1073/pnas.0504830102
发表时间：
2005-09-13
影响因子：
11.1
作者：
Crawford, PA;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
41.2
作者：
Lee, Yonghyun;Sugihara, Kohei;Moon, James J.
通讯作者：
Moon, James J.
粪便微生物群移植可防止辐射引起的毒性。
影响因子：
11.1
作者：
Cui M;Xiao H;Li Y;Zhou L;Zhao S;Luo D;Zheng Q;Dong J;Zhao Y;Zhang X;Zhang J;Lu L;Wang H;Fan S
通讯作者：
Fan S