Eosinophils and eosinophil cationic protein in nasal lavages in allergen‐induced hyperresponsiveness: effects of topical glucocorticosteroid treatment
Eosinophils and eosinophil cationic protein in nasal lavages in allergen‐induced hyperresponsiveness: effects of topical glucocorticosteroid treatment
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过敏原引起的高反应性中鼻腔灌洗液中的嗜酸性粒细胞和嗜酸性粒细胞阳离子蛋白：局部糖皮质激素治疗的效果
DOI：
--
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
U. Pipkorn
中科院分区：
文献类型：
--
作者：
M. Andersson;P. Andersson;P. Venge;U. Pipkorn
过敏原对呼吸道的挑战经常伴随着对特定和非特定刺激的反应性增加。下呼吸道的这种现象与对变应原挑战的晚期反应有关。晚期反应的特征之一是组织中粒细胞的渗透，特别是嗜酸性粒细胞。反应性增加的一个假说是，这些嗜酸性粒细胞局部激活，随后释放有毒颗粒成分，导致呼吸道上皮屏障功能下降。本研究的目的是进一步探讨这一假设。10名先前证实有鼻过敏原诱导高反应性的患者参加了一项双盲交叉研究，涉及不同时期外用糖皮质激素的预治疗。患者在第1天接受鼻内变应原激发，24小时后再次激发，并注意到反应性的增加。在激发前立即进行的鼻腔灌洗中，测定了嗜酸性粒细胞数量和嗜酸性阳离子蛋白(ECP)水平。正如预期的那样，与24小时前的初始变应原激发相比，接受安慰剂治疗的患者在再次激发时表现出鼻腔反应性的增强，并伴随着在再次激发前灌洗液中嗜酸性粒细胞和ECP增加的趋势。第二天灌洗液中ECP的含量与同一灌洗液中持续炎症活动的标志物驯化酯酶活性相关(r=0.64；P<；0.05)，提示嗜酸性粒细胞参与了对变应原的炎症反应。然而，第1天或第2天鼻腔灌洗液中的嗜酸性粒细胞数量和ECP含量与第2天获得的反应性增加的程度之间没有相关性。此外，糖皮质激素的局部治疗完全消除了增加的反应性，似乎没有显著改变初次或再次激发前鼻腔灌洗中的ECP水平。因此，我们认为呼吸道变应原激发后的反应性增加不仅是嗜酸性粒细胞激活的结果，而且是一种更复杂的现象。
Airway challenges with allergen are frequently followed by an increase in responsiveness to both specific and unspecific stimuli. This phenomenon in the lower airways has been linked to the presence of a late phase response to allergen challenge. One of the hallmarks of the late phase response is a tissue infiltration of granulocytes, specifically the eosinophils. A hypothesis for the increase in responsiveness has been that local activation of these eosinophils with subsequent release of the toxic granular constituents leads to a decrease in the barrier function of the airway epithelium. The aim of the present study was to further explore this hypothesis. Ten patients with previously demonstrated nasal allergen‐induced hyperresponsiveness participated in a double‐blind cross‐over study involving pretreatment for various periods with topical glucocorticosteroids. The patients were challenged intranasally with allergen on one day, rechallenge 24 h later and the increase in responsiveness noted. In a nasal lavage performed immediately prior to challenge the number of eosinophilic granulocutes and the level of eosinophilic cationic protein (ECP) was determined. As expected, the placebo‐treated patients demonstrated an increase in nasal responsiveness at rechallenge accompanied by a tendency for an increase in eosinophils as well as ECP in the lavage preceding the rechallenge compared with the initial allergen challenge 24 h previously. The amount of ECP found in the lavage on the second day correlated with the TAME‐esterase activity, a marker of ongoing inflammatory activity (r = 0.64; P<0.05) in the same lavage, suggesting participation of the eosinophilic granulocyte in the inflammatory response to allergen. However, there were no correlations between number of eosinophils or ECP content in the nasal lavages on the first or second day and the degree of the increased responsiveness obtained on the second day. Further, topical treatment with glucocorticosteroids, which totally abolished the increased responsiveness, did not appear to significantly change the levels of ECP in the nasal lavage prior to the initial or re‐challenge. It is therefore suggested that the increase in responsiveness seen after an airway allergen challenge is not only a result of eosinophil activation but is a more complex phenomenon.
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过敏原刺激鼻气道后介质释放。
DOI：
10.1164/arrd.1983.128.4.597
发表时间：
1983
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Naclerio,RM;Meier,HL;Kagey-Sobotka,A;AdkinsonJr,NF;Meyers,DA;Norman,PS;Lichtenstein,LM
通讯作者：
Lichtenstein,LM
过敏性鼻炎中嗜酸性粒细胞鲁米诺依赖性化学发光增强。
DOI：
10.1016/0091-6749(86)90414-8
发表时间：
1986
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Shult,PA;Graziano,FM;Busse,WW
通讯作者：
Busse,WW
DOI：
10.1164/ajrccm/138.2.406
发表时间：
1988-08-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
BASCOM, R;PIPKORN, U;NACLERIO, RM
通讯作者：
NACLERIO, RM
影响因子：
158.5
作者：
PIPKORN, U;PROUD, D;NACLERIO, RM
通讯作者：
NACLERIO, RM
影响因子：
158.5
作者：
NACLERIO, RM;PROUD, D;LICHTENSTEIN, LM
通讯作者：
LICHTENSTEIN, LM