Multinode acoustic focusing for parallel flow cytometry.
Multinode acoustic focusing for parallel flow cytometry.
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DOI：
10.1021/ac200963n
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发表时间：
2012-02-21
影响因子：
7.4
通讯作者：
Graves, Steven W.
中科院分区：
文献类型：
--
作者：
Piyasena, Menake E.;Suthanthiraraj, Pearlson P. Austin;Applegate, Robert W., Jr.;Goumas, Andrew M.;Woods, Travis A.;Lopez, Gabriel P.;Graves, Steven W.
流式细胞术可以同时测量和分析单个细胞或颗粒的多种性质，具有高灵敏度和精确度。然而，传统的流式细胞仪在分析大于约70微米的颗粒、以大于几百微升/分钟的流速进行分析以及提供大于50，000/秒的分析速率方面具有基本的限制。为了克服这些限制，我们开发了多节点声聚焦流动池，可以将颗粒（小到红细胞，大到直径107微米）定位到多达37个平行的流动流中。我们证明了这样的流动细胞的高通量，并行流式细胞仪的发展的潜力，通过精确聚焦的流式细胞仪对准微球，红细胞，和分析的CD 4+细胞免疫表型测定。这种方法将对创建用于稀有细胞检测应用（例如循环肿瘤细胞）、需要大颗粒分析的应用和高容量流式细胞术的高通量流式细胞仪产生重大影响。
Flow cytometry can simultaneously measure and analyze multiple properties of single cells or particles with high sensitivity and precision. Yet, conventional flow cytometers have fundamental limitations with regards to analyzing particles larger than about 70 microns, analyzing at flow rates greater than a few hundred microliters per minute, and providing analysis rates greater than 50,000 per second. To overcome these limits, we have developed multi-node acoustic focusing flow cells that can position particles (as small as a red blood cell and as large as 107 microns in diameter) into as many as 37 parallel flow streams. We demonstrate the potential of such flow cells for the development of high throughput, parallel flow cytometers by precision focusing of flow cytometry alignment microspheres, red blood cells, and the analysis of CD4+ cellular immunophenotyping assay. This approach will have significant impact towards the creation of high throughput flow cytometers for rare cell detection applications (e.g. circulating tumor cells), applications requiring large particle analysis, and high volume flow cytometry.
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影响因子：
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