Production of oligosaccharides and biofuels from Miscanthus using combinatorial steam explosion and ionic liquid pretreatment.
Production of oligosaccharides and biofuels from Miscanthus using combinatorial steam explosion and ionic liquid pretreatment.
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以芒属植物为原料，采用蒸汽爆炸和离子液体相结合的方法生产低聚糖和生物燃料。
DOI：
10.1016/j.biortech.2020.124625
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发表时间：
2021-03
影响因子：
11.4
通讯作者：
Gallagher JA
中科院分区：
文献类型：
--
作者：
Bhatia R;Lad JB;Bosch M;Bryant DN;Leak D;Hallett JP;Franco TT;Gallagher JA
对于低聚糖生产，建议进行SE和[C2 mim][OAM]预处理。90%（w/w）的初始生物质木聚糖通过[C2mim][0Ac]预处理溶解到XOS中。使用商业内切-1，4-β-D-葡聚糖酶将高达50%的葡聚糖转化为GOS。SE + [TEA][HSO 4]与[TEA][HSO 4]预处理相比，利润增加33%。低聚木糖有助于提高综合生物精炼工艺的经济可行性。预处理策略是有效解构木质纤维素生物质和经济地生产生物燃料、生物材料和生物基化学品的基础。本研究评估了单独和组合蒸汽爆破（SE）和离子液体（IL）预处理生产高价值的寡糖从一个新的种子为基础的芒杂交种（Mx2779）。用于预处理的两种离子液体是三乙基硫酸氢铵[TEA][HSO 4]和1-乙基-3-甲基咪唑鎓乙酸盐[C2mim][OAc]。结果表明，不同预处理对木质纤维素的破碎（纤维素和木聚糖溶解、脱木素、脱乙酰基）和物理化学改性（纤维素和木质素性质）均有不同的影响。这又决定了纤维素浆酶促水解成葡萄糖或葡萄糖低聚糖的效率，以用于下游应用。我们的研究结果表明，独立的SE或[C2mim][OAc]预处理可以通过生产低聚糖（如木寡糖和葡萄糖低聚糖）提供优于[TEA][HSO 4]的成本优势。这项研究还强调了与从芒草Mx2779生物质生产寡糖相关的技术和经济预处理工艺挑战。
For oligosaccharides production, SE and [C2mim][OAc] pretreatment is advised. 90% (w/w) of initial biomass xylan dissolved to XOS via [C2mim][OAc] pretreatment. Up to 50% glucan to GOS conversion using commercial endo-1,4-β-D-glucanases. 33% extra profit for SE + [TEA][HSO4] compared to [TEA][HSO4] pretreatment. XOS could contribute to economic viability of an integrated biorefining process. Pretreatment strategies are fundamental to effectively deconstruct lignocellulosic biomass and economically produce biofuels, biomaterials and bio-based chemicals. This study evaluated individual and combinatorial steam explosion (SE) and ionic liquid (IL) pretreatments for production of high-value oligosaccharides from a novel seed-based Miscanthus hybrid (Mx2779). The two ILs used for pretreatment were triethylammonium hydrogen sulphate [TEA][HSO4] and 1-ethyl-3-methylimidazolium acetate [C2mim][OAc]. The results showed that each pretreatment leads to distinct effects on the fragmentation (cellulose and xylan dissolution, delignification, deacetylation) and physicochemical modification (cellulose and lignin properties) of lignocellulose. This, in turn, dictated enzymatic hydrolysis efficiencies of the cellulose pulp to glucose or gluco-oligosaccharides for downstream applications. Our findings suggest that the stand-alone SE or [C2mim][OAc] pretreatments may offer cost advantages over [TEA][HSO4] through the production of oligosaccharides such as xylo- and gluco-oligosaccharides. This study also highlights technical and economic pretreatment process challenges related to the production of oligosaccharides from Miscanthus Mx2779 biomass.
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影响因子：
5.6
作者：
Hastings A;Mos M;Yesufu JA;McCalmont J;Schwarz K;Shafei R;Ashman C;Nunn C;Schuele H;Cosentino S;Scalici G;Scordia D;Wagner M;Clifton-Brown J
通讯作者：
Clifton-Brown J
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1.2
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