Expression of three essential antioxidants of Helicobacter pylori in clinical isolates
Expression of three essential antioxidants of Helicobacter pylori in clinical isolates
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幽门螺杆菌三种必需抗氧化剂在临床分离株中的表达
DOI：
10.1631/jzus.b1300171
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Ding, Shi-gang
中科院分区：
文献类型：
--
作者：
Chen, Mo;Zhang, Yue-xia;Zhang, Jing;Ding, Shi-gang
目的：幽门螺杆菌在宿主体内长期存在，并通过许多抗氧化蛋白对抗氧化应激，这些蛋白预计与细菌相关性胃病有关。我们的目的是研究三种必需的抗氧化剂在H。从具有不同临床结果的患者中分离的幽门螺杆菌菌株。方法：40例H.从13例胃癌、13例消化性溃疡和14例胃炎患者的胃粘膜内镜活检标本中分离出pylori菌株。硫氧还蛋白1（Trx 1）、硫氧还蛋白酶（RocF）和烷基过氧化氢还原酶（AhpC）在H. pylori用实时荧光定量PCR检测。使用单因素ANOVA检验分析多个样本集之间的比较。Pearson相关性检验用于评估多个连续变量之间的关系。结果：Trx 1在H. pylori在胃癌和消化性溃疡组织中的表达高于胃炎组织。RocF基因的表达。pylori在胃癌组织中的表达高于消化性溃疡和胃炎。然而，我们没有发现来自具有不同临床结果的患者的样本中AhpC表达的任何差异。Trx 1与RocF的表达呈正相关。Trx 1与AhpC表达呈正相关，但无线性趋势。RocF和AhpC的表达无相关性。结论：Trx 1和RocF在H. pylori可能与胃癌的发生有关。In H. pylori中，抗氧化系统成员的表达可能与胃癌相关。
Objective: Helicobacter pylori maintains long-term persistence in the host and combats oxidative stress via many antioxidant proteins, which are expected to be relevant to bacterial-associated gastric diseases. We aimed to investigate the expression of three essential antioxidants in H. pylori strains isolated from patients with different clinical outcomes. Methods: Forty H. pylori strains were isolated from endoscopic biopsy specimens of gastric mucosa from 13 patients with gastric cancer, 13 with peptic ulcer, and 14 with gastritis. The expression of thioredoxin 1 (Trx1), arginase (RocF), and alkyl hydroperoxide reductase (AhpC) in H. pylori was measured by real-time PCR. Comparisons among multiple sample sets were analyzed using a one-way ANOVA test. Pearson’s correlation test was used to assess relationships among multiple continuous variables. Results: Trx1 expression of H. pylori in gastric cancer and peptic ulcer tissues was higher than that in tissues with gastritis. RocF expression of H. pylori in gastric cancer tissues was higher than that in tissues exhibiting peptic ulcer and gastritis. However, we did not find any differences in AhpC expression in samples from patients with different clinical outcomes. The expression of Trx1 and RocF had a positive, linear correlation. The expression of Trx1 and AhpC had a positive correlation without a linear trend. We found no correlation between the expression of RocF and AhpC. Conclusions: Our observations indicate that the expression of Trx1 and RocF in H. pylori might be related to gastric carcinogenesis. In H. pylori, the expression of members of the antioxidant system may be correlated and relevant to gastric cancer.
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DOI：
10.1016/b978-0-12-374279-7.17002-x
发表时间：
2016
期刊：
--
影响因子：
--
作者：
Sushil Kumar;Christopher J. Chan;L. Coussens
通讯作者：
Sushil Kumar;Christopher J. Chan;L. Coussens
DOI：
10.1631/jzus.b1200102
发表时间：
2013-03
期刊：
Journal of Zhejiang University SCIENCE B
影响因子：
--
作者：
Hai-hong Zhang;A. Cai;Xue-ming Wei;L. Ding;Feng-zhi Li;Ai-ming Zheng;D. Dai;Rong-rong Huang;Hou-jun Cao;Haiyun Zhou;Jian-mei Wang;Xue-jing Wang;Wei Shi;Heng Zhu;Xiao-ying Yuan;Lin Chen
通讯作者：
Hai-hong Zhang;A. Cai;Xue-ming Wei;L. Ding;Feng-zhi Li;Ai-ming Zheng;D. Dai;Rong-rong Huang;Hou-jun Cao;Haiyun Zhou;Jian-mei Wang;Xue-jing Wang;Wei Shi;Heng Zhu;Xiao-ying Yuan;Lin Chen
DOI：
10.1002/9781118468869.ch4
发表时间：
2017-06
期刊：
--
影响因子：
--
作者：
P. Beatty;Sandra Cascio;Olivera J. Finn
通讯作者：
P. Beatty;Sandra Cascio;Olivera J. Finn
影响因子：
4.4
作者：
Zabaleta, J;McGee, DJ;Ochoa, AC
通讯作者：
Ochoa, AC
影响因子：
4.8
作者：
McGee, DJ;Kumar, S;Windle, HJ
通讯作者：
Windle, HJ