Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.
Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.
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DOI：
10.1371/journal.pone.0004119
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Vences M
中科院分区：
文献类型：
--
作者：
Aliabadian M;Kaboli M;Nijman V;Vences M
基于线粒体细胞色素氧化酶亚基I基因(cox1或coi)的DNA条形码已经成功地在广泛的分类群中进行了物种鉴定，但在某些情况下，未能划定密切相关的异地物种或杂交姐妹物种的物种边界。在这项研究中，我们扩大了先前对COX1(2776个序列，756个物种)的鸟类研究的样本量，并特别针对已知在全北极范围内发生在热带旁和/或已知的杂交的物种。为了获得更大的分类群(总共2719种)，我们还包括了另外两个线粒体基因的DNA序列：细胞色素b(Cob)(4614个序列，2087个物种)和16S(708个序列，498个物种)。我们的结果证实了Cox1和Cob的种内和种间差异存在很大差距，并表明基于距离的DNA条形码在所有可能的配对比较中提供了足够的信息来识别和描绘鸟类物种。这种DNA条形码差距在统计学上不受每个物种测序的个体数量的影响。然而，大多数杂交的近地物种对在Cox1和Cob的种内距离和种间距离之间存在平均分歧。因此，DNA条形码如果被用作物种发现的工具，将无法识别杂交的寄生物种对。然而，它们中的大多数可能仍然可以通过基于特征的方法归类到已知物种，尽管有必要开发互补的核标记来解释杂交物种中的线粒体导入。
DNA barcoding based on the mitochondrial cytochrome oxidase subunit I gene (cox1 or COI) has been successful in species identification across a wide array of taxa but in some cases failed to delimit the species boundaries of closely allied allopatric species or of hybridising sister species. In this study we extend the sample size of prior studies in birds for cox1 (2776 sequences, 756 species) and target especially species that are known to occur parapatrically, and/or are known to hybridise, on a Holarctic scale. In order to obtain a larger set of taxa (altogether 2719 species), we include also DNA sequences of two other mitochondrial genes: cytochrome b (cob) (4614 sequences, 2087 species) and 16S (708 sequences, 498 species). Our results confirm the existence of a wide gap between intra- and interspecies divergences for both cox1 and cob, and indicate that distance-based DNA barcoding provides sufficient information to identify and delineate bird species in 98% of all possible pairwise comparisons. This DNA barcoding gap was not statistically influenced by the number of individuals sequenced per species. However, most of the hybridising parapatric species pairs have average divergences intermediate between intraspecific and interspecific distances for both cox1 and cob. DNA barcoding, if used as a tool for species discovery, would thus fail to identify hybridising parapatric species pairs. However, most of them can probably still assigned to known species by character-based approaches, although development of complementary nuclear markers will be necessary to account for mitochondrial introgression in hybridising species.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
9.8
作者：
Hebert PD;Stoeckle MY;Zemlak TS;Francis CM
通讯作者：
Francis CM
影响因子：
56.9
作者：
GRANT, PR;GRANT, BR
通讯作者：
GRANT, BR
DOI：
10.1098/rstb.1986.0011
发表时间：
1986-01-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
AVISE, JC
通讯作者：
AVISE, JC
影响因子：
9.5
作者：
Kumar, S;Tamura, K;Nei, M
通讯作者：
Nei, M