Identification of Birds through DNA Barcodes.
Identification of Birds through DNA Barcodes.
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DOI：
10.1371/journal.pbio.0020312
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发表时间：
2004-10
期刊：
影响因子：
9.8
通讯作者：
Francis CM
中科院分区：
文献类型：
--
作者：
Hebert PD;Stoeckle MY;Zemlak TS;Francis CM
来自基因组标准化区域的短DNA序列提供了识别物种的DNA条形码。编制一个与命名标本链接的DNA条形码公共图书馆，可能会为识别物种提供一把新的万能钥匙，随着分类群覆盖率的增加，以及更快、更便宜的测序，这把钥匙的力量将会增强。最近的工作表明，线粒体基因648个碱基的序列多样性，即细胞色素C氧化酶I(COI)，可能作为一种DNA条形码用于动物物种的鉴定。这项研究测试了COI条形码在区分鸟类物种方面的有效性，鸟类是规模最大、研究最深入的脊椎动物群体之一。我们确定了260种北美鸟类的COI条形码，发现区分物种通常很简单。所有物种都有不同的COI条形码(S)，密切相关物种之间的差异平均是物种内差异的18倍。我们的结果确定了四个可能的北美鸟类新物种，这表明全球调查将导致许多其他鸟类的认识。与物种内的微小差异相比，物种间COI序列的巨大差异的发现证实了COI条形码在鸟类物种识别方面的有效性。这一结果加上其他动物群体的结果表明，标准的序列差异筛选阈值(10×种内平均差异)可能会加快新动物物种的发现。越来越多的证据表明DNA条形码作为物种鉴定的基础是有效的，这支持了一项国际活动，该活动最近开始汇编与命名标本相关联的COI序列的综合文库。单个基因能用来识别所有不同的动物物种吗？一项对255只北美鸟类的分析表明，细胞色素C氧化酶I可能具有这种潜力
Short DNA sequences from a standardized region of the genome provide a DNA barcode for identifying species. Compiling a public library of DNA barcodes linked to named specimens could provide a new master key for identifying species, one whose power will rise with increased taxon coverage and with faster, cheaper sequencing. Recent work suggests that sequence diversity in a 648-bp region of the mitochondrial gene, cytochrome c oxidase I (COI), might serve as a DNA barcode for the identification of animal species. This study tested the effectiveness of a COI barcode in discriminating bird species, one of the largest and best-studied vertebrate groups. We determined COI barcodes for 260 species of North American birds and found that distinguishing species was generally straightforward. All species had a different COI barcode(s), and the differences between closely related species were, on average, 18 times higher than the differences within species. Our results identified four probable new species of North American birds, suggesting that a global survey will lead to the recognition of many additional bird species. The finding of large COI sequence differences between, as compared to small differences within, species confirms the effectiveness of COI barcodes for the identification of bird species. This result plus those from other groups of animals imply that a standard screening threshold of sequence difference (10× average intraspecific difference) could speed the discovery of new animal species. The growing evidence for the effectiveness of DNA barcodes as a basis for species identification supports an international exercise that has recently begun to assemble a comprehensive library of COI sequences linked to named specimens. Can a single gene be used to identify all different animal species? An analysis of 255 North American birds shows that cytochrome c oxidase I might have this potential
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
2.8
作者：
Aleixo, A
通讯作者：
Aleixo, A
影响因子：
3.1
作者：
Grant, WS;Bowen, BW
通讯作者：
Bowen, BW
DOI：
10.1098/rspb.1998.0317
发表时间：
1998-03-22
影响因子：
4.7
作者：
Avise, JC;Walker, D
通讯作者：
Walker, D
影响因子：
2.8
作者：
Banks, RC;Cicero, C;Stotz, DF
通讯作者：
Stotz, DF