Protocols for Plasmodium gametocyte production in vitro: an integrative review and analysis.
Protocols for Plasmodium gametocyte production in vitro: an integrative review and analysis.
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DOI：
10.1186/s13071-022-05566-3
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发表时间：
2022-12-05
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
在体外生产疟原虫配子体是一个真正的挑战。已经描述了许多方案，但很少有方案能够产生足够数量的有活力和传染性的配子体，以进行但不限于传播阻断药物和疫苗开发的研究。这篇综述的目的是确定和讨论配子体生产方案已经发展了近二十年。我们根据文献检索和全面回顾分析了2000年以来发表的原始配子体生产方案。对PubMed、Web of Sciences和ScienceDirect数据库中的相关文章进行了系统综述。共发现23项关于疟原虫配子体产生的研究，其中19项涉及体外恶性疟原虫，1项涉及诺氏疟原虫，3项涉及离体间日疟原虫。在体外研究中，90%使用环境应激源来触发配子细胞发生。成熟配子细胞率高达4%。几个生物学参数有助于体外最佳生产有活力和传染性的成熟配子体。通过对配子细胞发生的分子机制的系统综述，我们可以建立具有可复制配子细胞的转基因寄生虫细胞系。这篇综述强调了对恶性疟原虫以外的其他疟原虫物种配子细胞生产方案的需求。
The production of Plasmodium gametocytes in vitro is a real challenge. Many protocols have been described, but few have resulted in the production of viable and infectious gametocytes in sufficient quantities to conduct research on—but not limited to—transmission-blocking drug and vaccine development. The aim of this review was to identify and discuss gametocyte production protocols that have been developed over the last two decades. We analyzed the original gametocyte production protocols published from 2000 onwards based on a literature search and a thorough review. A systematic review was performed of relevant articles identified in the PubMed, Web of Sciences and ScienceDirect databases. A total 23 studies on the production of Plasmodium gametocytes were identified, 19 involving in vitro Plasmodium falciparum, one involving Plasmodium knowlesi and three involving ex vivo Plasmodium vivax. Of the in vitro studies, 90% used environmental stressors to trigger gametocytogenesis. Mature gametocytemia of up to 4% was reported. Several biological parameters contribute to an optimal production in vitro of viable and infectious mature gametocytes. The knowledge gained from this systematic review on the molecular mechanisms involved in gametocytogenesis enables reproducible gametocyte protocols with transgenic parasite lines to be set up. This review highlights the need for additional gametocyte production protocols for Plasmodium species other than P. falciparum.
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影响因子：
5.7
作者：
通讯作者：
--
影响因子：
3.2
作者：
Demanga CG;Eng JWL;Gardiner DL;Roth A;Butterworth A;Adams JH;Trenholme KR;Dalton JP
通讯作者：
Dalton JP
影响因子：
2.1
作者：
Bates, Adam H.;Mu, Jianbing;Su, Xin-zhuan
通讯作者：
Su, Xin-zhuan
影响因子：
30.3
作者：
Gulati S;Ekland EH;Ruggles KV;Chan RB;Jayabalasingham B;Zhou B;Mantel PY;Lee MC;Spottiswoode N;Coburn-Flynn O;Hjelmqvist D;Worgall TS;Marti M;Di Paolo G;Fidock DA
通讯作者：
Fidock DA
影响因子：
14.8
作者：
Brancucci, Nicolas M. B.;Goldowitz, Ilana;Marti, Matthias
通讯作者：
Marti, Matthias