Improved healing of critical-size femoral defect in osteoporosis rat models using 3D elastin/polycaprolactone/nHA scaffold in combination with mesenchymal stem cells.
Improved healing of critical-size femoral defect in osteoporosis rat models using 3D elastin/polycaprolactone/nHA scaffold in combination with mesenchymal stem cells.
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DOI：
10.1007/s10856-021-06495-w
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发表时间：
2021-03-08
期刊：
影响因子：
--
通讯作者：
Abdollahifar MA
中科院分区：
文献类型：
--
作者：
Hejazi F;Ebrahimi V;Asgary M;Piryaei A;Fridoni MJ;Kermani AA;Zare F;Abdollahifar MA
骨质疏松症是一种常见的骨骼疾病，导致骨折的风险增加，并且这种疾病涉及延迟的骨愈合和受损的骨再生。在这项研究中，弹性蛋白/聚己内酯/nHA纳米纤维支架与间充质干细胞组合用于再生骨缺损。在体外评价细胞毒性、细胞相容性和细胞形态，观察结果表明，该支架为细胞附着、生长、迁移和增殖提供了适当的环境。在卵巢切除（OVX）后3个月，建立大鼠股骨临界缺损动物模型，评价骨髓间充质干细胞（BM-MSCs）在三维支架上的成骨分化潜能。在本实验研究中，将24只雌性Wistar大鼠等分为三组：对照组、支架组（未接种BM-MSC）和支架+细胞组（接种BM-MSC）。手术后30天，取出右侧股骨，进行体视学分析和RNA提取，以检测Bmp-2和VEGF基因的表达。结果显示支架组和支架+细胞组的体视学参数和Bmp-2、VEGF的表达较对照组显著增加。目前的研究表明，使用三维弹性蛋白/聚己内酯（PCL）/纳米羟基磷灰石（nHA）支架与MSCs组合可以改善大鼠骨折再生，加速截骨部位的骨愈合。
Osteoporosis is a common bone disease that results in elevated risk of fracture, and delayed bone healing and impaired bone regeneration are implicated by this disease. In this study, Elastin/Polycaprolactone/nHA nanofibrous scaffold in combination with mesenchymal stem cells were used to regenerate bone defects. Cytotoxicity, cytocompatibility and cellular morphology were evaluated in vitro and observations revealed that an appropriate environment for cellular attachment, growth, migration, and proliferation is provided by this scaffold. At 3 months following ovariectomy (OVX), the rats were used as animal models with an induced critical size defect in the femur to evaluate the therapeutic potential of osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) seeded on 3 dimension (3D) scaffolds. In this experimental study, 24 female Wistar rats were equally divided into three groups: Control, scaffold (non-seeded BM-MSC), and scaffold + cell (seeded BM-MSC) groups. 30 days after surgery, the right femur was removed, and underwent a stereological analysis and RNA extraction in order to examine the expression of Bmp-2 and Vegf genes. The results showed a significant increase in stereological parameters and expression of Bmp-2 and Vegf in scaffold and scaffold + cell groups compared to the control rats. The present study suggests that the use of the 3D Elastin/Polycaprolactone (PCL)/Nano hydroxyapatite (nHA) scaffold in combination with MSCs may improve the fracture regeneration and accelerates bone healing at the osteotomy site in rats.
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