Nobiletin suppresses the development of experimental autoimmune encephalomyelitis mediated by modulation of T helper 17 cell differentiation.
Nobiletin suppresses the development of experimental autoimmune encephalomyelitis mediated by modulation of T helper 17 cell differentiation.
复制标题
川陈皮素通过调节辅助性T细胞17（T helper 17）的分化，抑制实验性自身免疫性脑脊髓炎的发展。
DOI：
10.3164/jcbn.20-178
复制
发表时间：
2021-09
影响因子：
2.4
通讯作者：
Sakai T
中科院分区：
文献类型：
--
作者：
Nakamoto A;Hirabayashi Y;Anzaki C;Nakamoto M;Shuto E;Sakai T
多发性硬化症是一种器官特异性自身免疫性疾病，靶向中枢神经系统中的髓鞘抗原。Nobiletin是一种在柑橘类水果中发现的膳食多甲氧基黄酮。在这项研究中，我们调查了诺贝尔如何影响疾病状态和髓鞘少突胶质细胞糖蛋白的免疫反应，在实验性自身免疫性脑脊髓炎小鼠。从免疫前14天至实验结束口服诺必列汀，并测定临床评分。在用髓鞘少突胶质细胞糖蛋白刺激的脾细胞的培养上清液中测量白细胞介素-17A和干扰素-γ的产生水平。此外，进行流式细胞术分析以检查川陈皮素对体外T细胞分化的影响。给药诺必利显著降低临床评分和脾细胞中白细胞介素-17A的产生。此外，体外分析显示，川陈皮素以剂量依赖性方式显著抑制Th 17细胞分化和白细胞介素-17A产生。结果表明，诺必利通过调节Th 17细胞分化来减轻实验性自身免疫性脑脊髓炎的严重程度。
Multiple sclerosis is an organ-specific autoimmune disease that targets the myelin antigen in the central nervous system. Nobiletin is a dietary polymethoxylated flavonoid found in citrus fruits. In this study, we investigated how nobiletin affects the disease state and immune responses to myelin oligodendrocyte glycoprotein in experimental autoimmune encephalomyelitis mice. Nobiletin was administered orally from 14 days before immunization until the end of the experiment, and clinical scores were determined. The production levels of interleukin-17A and interferon-γ were measured in a culture supernatant of splenocytes stimulated with myelin oligodendrocyte glycoprotein. In addition, flow cytometric analysis was performed to examine the effect of nobiletin on T cell differentiation in vitro. Administration of nobiletin significantly decreased the clinical score and interleukin-17A production in splenocytes. Furthermore, in vitro analysis showed that nobiletin significantly suppressed Th17 cell differentiation and interleukin-17A production in a dose-dependent manner. The results suggest that nobiletin attenuates experimental autoimmune encephalomyelitis severity through modulation of Th17 cell differentiation.
登录
查看更多内容
影响因子：
64.5
作者：
Ivanov, Ivaylo I.;McKenzie, Brent S.;Littman, Dan R.
通讯作者：
Littman, Dan R.
影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
影响因子：
6
作者：
Tzartos, John S.;Friese, Manuel A.;Fugger, Lars
通讯作者：
Fugger, Lars
影响因子：
4.4
作者：
Heo, Yu-Jung;Joo, Young-Bin;Min, Jun-Ki
通讯作者：
Min, Jun-Ki
影响因子：
5.8
作者：
Matusevicius, D;Kivisäkk, P;Link, H
通讯作者：
Link, H