Ethanol concentration-dependent alterations in gene expression during acute binge drinking in the HIV-1 transgenic rat.
Ethanol concentration-dependent alterations in gene expression during acute binge drinking in the HIV-1 transgenic rat.
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DOI：
10.1111/acer.12077
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发表时间：
2013-07
期刊：
影响因子：
--
通讯作者：
Chang SL
中科院分区：
文献类型：
--
作者：
Sarkar S;Chang SL
酗酒高乙醇（EtOH）浓度饮料在年轻人中很常见，可能是暴露于性传播疾病（包括HIV-1）的风险因素。我们使用了一种新型的非感染性HIV-1转基因（HIV-1 Tg）大鼠模型，该模型模拟HIV-1患者的免疫反应改变和认知学习和记忆缺陷，以研究乙醇浓度依赖性对48个酒精调节基因的影响。HIV-1 Tg和对照F344大鼠通过管饲法（i.g.）连续3天（2.0 g/kg/d）。末次给药后2小时，采集每只动物的血液、肝脏和脾脏。测量血清血液EtOH浓度（BEC），并使用专门设计的PCR阵列测定肝脏和脾脏中的基因表达。与8% EtOH组相比，52% EtOH处理的HIV-1 Tg大鼠的BEC显著更高;然而，与52% EtOH组相比，8% EtOH处理的对照大鼠的BEC更高。在8或52%EtOH处理的HIV-1 Tg大鼠中，EtOH代谢相关基因Adh 1、Adh 4和Cyp 2 e1的表达没有变化，而在52%EtOH对照大鼠的肝脏中这些基因的表达显著升高，但在8%EtOH组中没有变化。在HIV-1 Tg大鼠中，GABAA、代谢型谷氨酸和多巴胺神经递质受体基因的表达在52%EtOH组的脾脏中显著增加，但在8%EtOH组中没有增加，而在任何对照组中均未观察到这些基因的变化。我们的数据表明，在存在HIV-1感染的情况下，乙醇浓度依赖性酗酒可能会产生显着不同的分子效应。
Binge drinking of high ethanol (EtOH) concentration beverages is common among young adults and can be a risk factor for exposure to sexually transmitted diseases, including HIV-1. We used a novel noninfectious HIV-1 transgenic (HIV-1Tg) rat model that mimics HIV-1 patients in terms of altered immune responses and deficits in cognitive learning and memory to investigate EtOH concentration-dependent effects on 48 alcohol-modulated genes during binge EtOH administration. HIV-1Tg and control F344 rats were administered water, 8% EtOH, or 52% EtOH by gavage (i.g.) for 3 days (2.0 g/kg/d). Two hours after final treatment, blood, liver, and spleen were collected from each animal. Serum blood EtOH concentration (BEC) was measured, and gene expression in the liver and spleen was determined using a specifically designed PCR array. The BEC was significantly higher in the 52% EtOH-treated HIV-1Tg rats compared with the 8% EtOH group; however, the BEC was higher in the 8% EtOH-treated control rats compared with the 52% EtOH group. There was no change in expression of the EtOH metabolism-related genes, Adh1, Adh4, and Cyp2e1, in either the 8 or 52% EtOH-treated HIV-1Tg rats, whereas expression of those genes was significantly higher in the liver of the 52% EtOH control rats, but not in the 8% EtOH group. In the HIV-1Tg rats, expression of the GABAA, metabotropic glutamate, and dopamine neurotransmitter receptor genes was significantly increased in the spleen of the 52% EtOH group, but not in the 8% EtOH group, whereas no change was observed in those genes in either of the control groups. Our data indicate that, in the presence of HIV-1 infection, EtOH concentration-dependent binge drinking can have significantly different molecular effects.
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影响因子：
3.3
作者：
Peng, Jinsong;Vigorito, Michael;Chang, Sulie L.
通讯作者：
Chang, Sulie L.
影响因子：
--
作者：
Samet, JH;Freedberg, KA;Hingson, R
通讯作者：
Hingson, R
DOI：
10.1073/pnas.161290298
发表时间：
2001-07-31
影响因子：
11.1
作者：
Reid, W;Sadowska, M;Bryant, J
通讯作者：
Bryant, J
影响因子：
1.5
作者：
Baum, Marianna K.;Rafie, Carlin;Campa, Adriana
通讯作者：
Campa, Adriana
影响因子：
5.3
作者：
Flora, G;Pu, H;Toborek, M
通讯作者：
Toborek, M