Network and Evolutionary Analysis Reveals Candidate Genes of Membrane Trafficking Involved in Maize Seed Development and Immune Response.
Network and Evolutionary Analysis Reveals Candidate Genes of Membrane Trafficking Involved in Maize Seed Development and Immune Response.
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网络和进化分析揭示了与玉米种子发育和免疫反应有关的膜运输候选基因。
DOI：
10.3389/fpls.2022.883961
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发表时间：
2022
影响因子：
5.6
通讯作者：
Li, Faqiang
中科院分区：
文献类型：
--
作者：
Zheng, Chunyan;Yu, Yin;Deng, Guiling;Li, Hanjie;Li, Faqiang
关键词：
植物膜转运系统在维持细胞正常功能和响应各种发育和环境信号方面起着至关重要的作用。到目前为止，我们对玉米膜运输系统的了解仍然有限。在本研究中，我们利用正交法从玉米基因组中系统地鉴定了479个膜转运基因，并结合转录组和进化分析研究了它们的功能。这些基因编码包被囊泡、AP复合体、自噬、ESCRT、逆转录因子、Rab GTP酶、拴系因子和圈套的成分。玉米基因表现出多样化但协调的表达模式，249个基因在生殖组织中表达上调。进一步的WGCNA分析表明，5个COPII组分和4个Rab GTP酶与胚乳发育过程中的蛋白质生物合成具有高度的连接性，自噬、ESCRT、Rab和SNARE的8个组分与防御相关基因和/或次生代谢过程强烈上调，从而赋予了对禾谷镰刀菌的基础抗性。此外，我们还鉴定了39个在玉米驯化和/或改良过程中具有较强选择信号的膜转运基因。其中，ZmSec23a和ZmVPS37A分别用于改良期间的籽粒产油量和驯化期间的抗病性。综上所述，这些发现将为未来了解膜运输基因在玉米中的功能提供重要的提示。
The plant membrane-trafficking system plays a crucial role in maintaining proper cellular functions and responding to various developmental and environmental cues. Thus far, our knowledge of the maize membrane-trafficking system is still limited. In this study, we systematically identified 479 membrane-trafficking genes from the maize genome using orthology search and studied their functions by integrating transcriptome and evolution analyses. These genes encode the components of coated vesicles, AP complexes, autophagy, ESCRTs, retromers, Rab GTPases, tethering factors, and SNAREs. The maize genes exhibited diverse but coordinated expression patterns, with 249 genes showing elevated expression in reproductive tissues. Further WGCNA analysis revealed that five COPII components and four Rab GTPases had high connectivity with protein biosynthesis during endosperm development and that eight components of autophagy, ESCRT, Rab, and SNARE were strongly co-upregulated with defense-related genes and/or with secondary metabolic processes to confer basal resistance to Fusarium graminearum. In addition, we identified 39 membrane-trafficking genes with strong selection signals during maize domestication and/or improvement. Among them, ZmSec23a and ZmVPS37A were selected for kernel oil production during improvement and pathogen resistance during domestication, respectively. In summary, these findings will provide important hints for future appreciation of the functions of membrane-trafficking genes in maize.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Schulze-Lefert, P