Antioxidant Effects of a Hydroxytyrosol-Based Pharmaceutical Formulation on Body Composition, Metabolic State, and Gene Expression: A Randomized Double-Blinded, Placebo-Controlled Crossover Trial.
Antioxidant Effects of a Hydroxytyrosol-Based Pharmaceutical Formulation on Body Composition, Metabolic State, and Gene Expression: A Randomized Double-Blinded, Placebo-Controlled Crossover Trial.
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基于羟基羟基醇的药物配方对人体组成，代谢状态和基因表达的抗氧化作用：一项随机的双盲，安慰剂对照的交叉试验。
DOI：
10.1155/2017/2473495
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发表时间：
2017
影响因子：
--
通讯作者：
De Lorenzo A
中科院分区：
文献类型：
--
作者：
Colica C;Di Renzo L;Trombetta D;Smeriglio A;Bernardini S;Cioccoloni G;Costa de Miranda R;Gualtieri P;Sinibaldi Salimei P;De Lorenzo A
羟基酪醇（HT）在心血管疾病（CVD）保护中起着重要作用，其代谢产物能够保护动脉粥样硬化中常见的内皮功能障碍。这项随机双盲、安慰剂对照交叉试验确定了健康志愿者服用两种含15 mg/天HT的胃耐药胶囊3周（HTT）的效果。对营养状况、血清代谢物、氧化应激生物标志物以及与氧化应激、炎症和心血管疾病相关的9个基因的基因表达进行了评价。氧化生物标志物，如巯基（p = 0.001），总抗氧化状态（TAS）（p = 0.001），超氧化物歧化酶1（SOD 1）（2-ΔΔCt = 3.7）和HT的血浆浓度（2.83 μg·mL−1）显著增加，而HTT后亚硝酸盐（p = 0.001）、硝酸盐（p = 0.001）和丙二醛（MDA）（p = 0.02）显著降低。HTT后观察到体脂质量百分比（p = 0.01）、髂上皮褶厚度（p = 0.01）和体重（p = 0.04; Δ% =-0.46%）显著降低。这项研究表明，定期摄入15毫克/天的HT改变了身体组成参数，并调节了抗氧化剂的特性以及炎症和氧化应激相关基因的表达。然而，建议个性化HT剂量，以发挥其在CVD预防和保护LDL-C颗粒免受氧化损伤方面的健康益处。本试验注册于ClinicalTrials.gov NCT 01890070。
Hydroxytyrosol (HT) plays a significant role in cardiovascular disease (CVD) protection, and its metabolites are able to protect from the endothelial dysfunction commonly present in atherosclerosis. This randomized double-blinded, placebo-controlled crossover trial determined the effect in healthy volunteers of two gastroresistant capsules containing 15 mg/day of HT, for a 3-week period (HTT). Evaluation of nutritional status, serum metabolites, oxidative stress biomarkers, and gene expression of 9 genes related to oxidative stress, inflammation, and CVDs was performed. Oxidation biomarkers like thiol group (p = 0.001), total antioxidant status (TAS) (p = 0.001), superoxide dismutase 1 (SOD1) (2−ΔΔCt = 3.7), and plasma concentration of HT (2.83 μg·mL−1) were significantly increased, while nitrite (p = 0.001), nitrate (p = 0.001), and malondialdehyde (MDA) (p = 0.02) were drastically reduced after HTT. A significant reduction of body fat mass percentage (p = 0.01), suprailiac skinfold (p = 0.01), and weight (p = 0.04; Δ% = −0.46%) was observed after HTT. This study shows that regular intake of 15 mg/day of HT changed body composition parameters and modulated the antioxidant profile and the expression of inflammation and oxidative stress-related genes. However, it is advisable to personalize HT doses in order to exert its health benefits in CVD prevention and protection of LDL-C particles from oxidative damage. This trial is registered with ClinicalTrials.gov NCT01890070.
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影响因子：
5.6
作者：
D'Archivio M;Filesi C;Varì R;Scazzocchio B;Masella R
通讯作者：
Masella R
影响因子：
3.3
作者：
Agostoni, Carlo;Bresson, Jean-Louis;Verhagen, Hans
通讯作者：
Verhagen, Hans
影响因子：
7.4
作者：
Covas, MI;de la Torre, K;de la Torre, R
通讯作者：
de la Torre, R
影响因子：
6.1
作者：
de la Puerta, R;Domínguez, MEM;Hoult, JRS
通讯作者：
Hoult, JRS
影响因子：
7.1
作者：
Bouwens, Mark;Afman, Lydia A.;Muller, Michael
通讯作者：
Muller, Michael