Profiling of differentially expressed genes in sheep T lymphocytes response to an artificial primary Haemonchus contortus infection.
Profiling of differentially expressed genes in sheep T lymphocytes response to an artificial primary Haemonchus contortus infection.
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绵羊 T 淋巴细胞对人工原发性捻转血矛线虫感染反应中差异表达基因的分析
DOI：
10.1186/s13071-015-0844-z
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发表时间：
2015-04-18
影响因子：
3.2
通讯作者：
Du AF
中科院分区：
文献类型：
--
作者：
Yang Y;Zhou QJ;Chen XQ;Yan BL;Guo XL;Zhang HL;Du AF
背景：扭曲血蜱是一种常见的吸血线虫，在农业中造成广泛的经济损失。弓形虫感染后，引起一系列宿主反应，特别是与T淋巴细胞免疫有关的反应。现有的研究主要集中在羊T淋巴细胞对扭曲弧菌的一般免疫应答上，缺乏分子水平的研究。本研究的目的是获得T淋巴细胞的系统转录谱在弯形螺旋体原发感染羊。方法：对无线虫的绵羊进行1次口腔感染。分别从感染后0、3、30和60 d的绵羊外周血中采集T淋巴细胞样本。微阵列用于比较样本之间的基因转录水平。采用定量RT-PCR验证芯片数据。利用基因本体和KEGG通路分析对差异表达基因进行标注。结果：我们的芯片数据与qPCR结果一致。通过微阵列分析，3d组与0d组、30d组与0d组、60d组与0d组分别获得853个、242个和42个差异表达基因。基因本体和KEGG通路分析表明，这些基因参与代谢、信号、细胞生长和免疫系统过程。SLC9A3R2、ABCB9、COMMD4、SUGT1、FCER1G、GSK3A、PAK4和FCER2等显著差异表达基因的功能分析揭示了它们与细胞稳态维持和免疫应答的重要关联。我们的数据表明，维持有效的免疫反应和自然细胞活性对T淋巴细胞对抗弯状芽孢杆菌感染很重要。结论：我们的研究结果提供了绵羊T淋巴细胞对弯曲弧菌感染反应的大量候选基因列表，并为感染后的一般免疫反应提供了新的见解。
Background:Haemonchus contortus is a common bloodsucking nematode causing widespread economic loss in agriculture. Upon H. contortus infection, a series of host responses is elicited, especially those related to T lymphocyte immunity. Existing studies mainly focus on the general immune responses of sheep T lymphocyte to H. contortus, lacking investigations at the molecular level. The objective of this study was to obtain a systematic transcriptional profiling of the T lymphocytes in H. contortus primary-infected sheep.Methods:Nematode-free sheep were orally infected once with H. contortus L3s. T lymphocyte samples were collected from the peripheral blood of 0, 3, 30 and 60 days post infection (dpi) infected sheep. Microarrays were used to compare gene transcription levels between samples. Quantitative RT-PCR was employed to validate the microarray data. Gene Ontology and KEGG pathway analysis were utilized for the annotation of differentially expressed genes.Results:Our microarray data was consistent with qPCR results. From microarrays, 853, 242 and 42 differentially expressed genes were obtained in the 3d vs. 0d, 30d vs. 0d and 60d vs. 0d comparison groups, respectively. Gene Ontology and KEGG pathway analysis indicated that these genes were involved in metabolism, signaling, cell growth and immune system processes. Functional analysis of significant differentially expressed genes, such as SLC9A3R2, ABCB9, COMMD4, SUGT1, FCER1G, GSK3A, PAK4 and FCER2, revealed a crucial association with cellular homeostasis maintenance and immune response. Our data suggested that maintaining both effective immunological response and natural cellular activity are important for T lymphocytes in fighting against H. contortus infection.Conclusions:Our results provide a substantial list of candidate genes in sheep T lymphocytes response to H. contortus infection, and contribute novel insights into a general immune response upon infection.
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影响因子：
--
作者：
Caughey, George H.
通讯作者：
Caughey, George H.
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
4
作者：
Andronicos, Nicholas;Hunt, Peter;Windon, Ross
通讯作者：
Windon, Ross
影响因子：
11.4
作者：
Abo, A;Qu, J;Minden, A
通讯作者：
Minden, A
影响因子：
82.9
作者：
Fox, JG;Beck, P;Nagler-Anderson, C
通讯作者：
Nagler-Anderson, C