MoMip11, a MoRgs7-interacting protein, functions as a scaffolding protein to regulate cAMP signaling and pathogenicity in the rice blast fungus Magnaporthe oryzae.
MoMip11, a MoRgs7-interacting protein, functions as a scaffolding protein to regulate cAMP signaling and pathogenicity in the rice blast fungus Magnaporthe oryzae.
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MoMip11 是一种 MoRgs7 相互作用蛋白，在稻瘟病菌 Magnaporthe oryzae 中作为支架蛋白调节 cAMP 信号传导和致病性
DOI：
10.1111/1462-2920.14102
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发表时间：
2018-09
影响因子：
5.1
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Yin Z;Zhang X;Wang J;Yang L;Feng W;Chen C;Gao C;Zhang H;Zheng X;Wang P;Zhang Z
稻瘟病真菌 Magnaporthe oryzae 具有 8 个 G 蛋白信号传导 (RGS) 和 RGS 样蛋白（MoRgs1 至 MoRgs8）调节因子，它们在真菌的生长、分化和致病性中表现出独特和共同的调节功能。我们发现MoRgs7具有独特的RGS-七跨膜（7-TM）结构域基序，在早期附着细胞分化过程中定位于高度动态的小管-囊泡区室，随后逐渐降解。为了探讨这是否涉及 MoRgs7 的主动信号感知，我们在米霉 MoMip11 中鉴定了与 MoRgs7 相互作用的 Gbeta 样/RACK1 蛋白同源物。有趣的是，MoMip11 选择性地与 cAMP 调节途径的多个组件相互作用，包括 Gα MoMagA 和高亲和力磷酸二酯酶 MoPdeH。我们进一步表明 MoMip11 促进 MoMagA 激活并抑制 MoPdeH 活性，从而上调细胞内 cAMP 水平。此外，MoMip11 是响应多种应激所必需的，Gbeta 样/RACK1 衔接蛋白也具有这一作用。总之，我们揭示了 MoMip11 连接 MoRgs7 和 G 蛋白以调节 cAMP 信号、应激反应和米霉致病性的独特机制。我们的研究揭示了控制稻瘟病这一重要病原体生长、发育和致病性的众多调控网络。
The rice blast fungus Magnaporthe oryzae has eight regulators of G-protein signaling (RGS) and RGS-like proteins (MoRgs1 to MoRgs8) that exhibit both distinct and shared regulatory functions in the growth, differentiation, and pathogenicity of the fungus. We found MoRgs7 with a unique RGS-seven transmembrane (7-TM) domain motif is localized to the highly dynamic tubule-vesicular compartments during early appressorium differentiation followed by gradually degradation. To explore whether this involves an active signal perception of MoRgs7, we identified a Gbeta-like/RACK1 protein homolog in M. oryzae MoMip11 that interacts with MoRgs7. Interestingly, MoMip11 selectively interacted with several components of the cAMP regulatory pathway, including Gα MoMagA and the high-affinity phosphodiesterase MoPdeH. We further showed that MoMip11 promotes MoMagA activation and suppresses MoPdeH activity thereby up-regulating intracellular cAMP levels. Moreover, MoMip11 is required for the response to multiple stresses, a role also shared by Gbeta-like/RACK1 adaptor proteins. In summary, we revealed a unique mechanism by which MoMip11 links MoRgs7 and G-proteins to reugulate cAMP signaling, stress responses, and pathogenicity of M. oryzae. Our studies revealed the multitude of regulatory networks that govern growth, development, and pathogenicity in this important causal agent of rice blast.
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影响因子：
64.8
作者：
Cheng, Zhenyu;Li, Jian-Feng;Niu, Yajie;Zhang, Xue-Cheng;Woody, Owen Z.;Xiong, Yan;Djonovic, Slavica;Millet, Yves;Bush, Jenifer;McConkey, Brendan J.;Sheen, Jen;Ausubel, Frederick M.
通讯作者：
Ausubel, Frederick M.
影响因子：
5.3
作者：
Chang, BY;Conroy, KB;Cartwright, CA
通讯作者：
Cartwright, CA
影响因子：
4.8
作者：
Liliental, J;Chang, DD
通讯作者：
Chang, DD
DOI：
10.1073/pnas.0509763102
发表时间：
2006-01-03
影响因子：
11.1
作者：
Gibson, SK;Gilman, AG
通讯作者：
Gilman, AG
影响因子：
11.6
作者：
Choi, WB;Dean, RA
通讯作者：
Dean, RA