Respiratory syncytial virus infection of microglia exacerbates SH-SY5Y neuronal cell injury by inducing the secretion of inflammatory cytokines: A Transwell in vitro study.
Respiratory syncytial virus infection of microglia exacerbates SH-SY5Y neuronal cell injury by inducing the secretion of inflammatory cytokines: A Transwell in vitro study.
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呼吸道合胞病毒感染小胶质细胞通过诱导炎性细胞因子的分泌加重SH-SY5Y神经元细胞损伤：Transwell体外研究
DOI：
10.22038/ijbms.2020.49193.11263
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发表时间：
2021-03
影响因子：
2.2
通讯作者：
Huang SH
中科院分区：
文献类型：
--
作者：
Zhang XY;Zhang XC;Yu HY;Wang Y;Chen J;Wang Y;Yu L;Zhu GX;Cao XJ;Huang SH
阐明呼吸道合胞病毒（RSV）感染和中枢神经元疾病的机制，并了解小胶质细胞在RSV感染过程中神经元损伤中的作用。 基于体外Transwell共培养系统评估RSV和RSV感染的CHME-5小胶质细胞产生的细胞因子对SY5Y神经元细胞的影响。本研究设立5个治疗组,包括正常对照SY5Y组、RSV+SY5Y感染组、(细胞因子+CHME-5)+SY5Y Transwell组、(RSV+CHME-5)+SY5Y Transwell组、(RSV+细胞因子+CHME-5)+SY5Y Transwell组。通过共聚焦显微镜分析 SY5Y 细胞及其突触的形态和物理变化。通过 qRT-PCR 和 Western blot 测定小胶质细胞中 TLR3/RIG-I 的 mRNA 和蛋白表达水平以及 Hv1 的表达。此外，通过流式细胞术测定神经元细胞的凋亡率。 RSV感染体外激活小胶质细胞Hv1蛋白表达（P<0.05），诱导SY5Y细胞形态改变，突触延长（73.36±0.12 μm vs 38.10±0.11 μm），同时激活TLR3和RIG-I蛋白表达（P<0.05），上调炎症细胞因子TNF-α、IL-6和IL-8的分泌(P<0.01)，并增加SY5Y细胞凋亡率(P<0.01)。结果表明，小胶质细胞的 RSV 感染可诱导 SY5Y 神经元细胞损伤，并通过炎症细胞因子释放刺激细胞凋亡。
To elucidate the mechanism of Respiratory Syncytial Virus (RSV) infection and central neuronal disease and to understand the role of microglia in neuronal injuries during RSV infection. The effects of RSV and the cytokines produced by RSV-infected CHME-5 microglial cells on SY5Y neuronal cells were evaluated based on an in vitro Transwell coculture system. Five treatment groups were established in this study, including the normal control SY5Y group, RSV+SY5Y infection group, (cytokine+CHME-5)+SY5Y Transwell group, (RSV+CHME-5)+SY5Y Transwell group, and (RSV+cytokine+CHME-5)+SY5Y Transwell group. The morphological and physical alterations in SY5Y cells and their synapses were analyzed by confocal microscopy. The mRNA and protein expression levels of TLR3/RIG-I, as well as the expression of Hv1, in microglia were measured by qRT-PCR and Western blot assays. In addition, the apoptosis ratio of neuronal cells was determined by flow cytometry. RSV infection activated the protein expression of Hv1 protein in microglia in vitro (P<0.05), induced morphological changes in SY5Y cells, lengthened synapses (73.36±0.12 μm vs 38.10±0.11 μm), simultaneously activated TLR3 and RIG-I protein expression (P<0.05), upregulated the secretion of the inflammatory cytokines TNF-α, IL-6, and IL-8 (P<0.01), and increased the apoptosis rate of SY5Y cells (P<0.01). The results demonstrate that RSV infection of microglia can induce SY5Y neuronal cell injury and stimulate apoptosis through inflammatory cytokine release.
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影响因子：
9
作者：
Bernardo, A. R.;Cosgaya, J. M.;Aranda, A.;Jimenez-Lara, A. M.
通讯作者：
Jimenez-Lara, A. M.
影响因子：
29.7
作者：
Nayak D;Roth TL;McGavern DB
通讯作者：
McGavern DB
小胶质细胞 Hv1 质子通道通过氧化损伤促进铜宗诱导的脱髓鞘。
影响因子：
4.7
作者：
Liu J;Tian D;Murugan M;Eyo UB;Dreyfus CF;Wang W;Wu LJ
通讯作者：
Wu LJ
影响因子：
2.2
作者：
Kawashima, Hisashi;Kashiwagi, Yasuyo;Kato, Yuichi
通讯作者：
Kato, Yuichi
影响因子：
3.6
作者：
Miyazaki, Kyohei;Hashimoto, Koichi;Hosoya, Mitsuaki
通讯作者：
Hosoya, Mitsuaki