Near-infrared lymphatic imaging demonstrates the dynamics of lymph flow and lymphangiogenesis during the acute versus chronic phases of arthritis in mice.
Near-infrared lymphatic imaging demonstrates the dynamics of lymph flow and lymphangiogenesis during the acute versus chronic phases of arthritis in mice.
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DOI：
10.1002/art.27464
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发表时间：
2010-07
影响因子：
--
通讯作者：
Xing, Lianping
中科院分区：
文献类型：
--
作者：
Zhou, Quan;Wood, Ronald;Schwarz, Edward M.;Wang, Yong-Jun;Xing, Lianping
建立评价K/B×N小鼠炎性关节炎模型淋巴引流功能的体内成像方法。将近红外(NIR)荧光染料吲哚青绿(ICG)皮内注射到野生型小鼠的足底，用806 nm近红外激光照射肢体，用CCD摄像机记录ICG从注射部位到引流窝淋巴结(PLN)的运动。对ICG-NIR图像进行分析，获得随时间变化的5项淋巴功能指标。K/B×N关节炎小鼠和对照非关节炎小鼠在1个月龄时开始急性关节炎症时进行成像，并在关节炎症变为慢性时3个月时重复成像。免疫组织化学检测PLN淋巴管生成情况。ICG及其在淋巴管内的运输很容易被可视化，并得到定量测量结果。在关节炎急性期，淋巴管扩张，ICG信号增强，淋巴脉冲增强，PLN迅速变成荧光。在慢性期，新的淋巴管出现在足部附近。然而，ICG在淋巴管中的出现延迟。K/B×N小鼠PLN淋巴窦的大小和面积逐渐增大。ICG-NIR淋巴显像是评价炎性关节炎小鼠淋巴引流功能的有价值的方法。在K/B×N小鼠的ICG-NIR成像中，在炎症的急性期和慢性期发现了两种不同的淋巴表型。这项技术可以用来评估淋巴疾病的新疗法。
Development of an in vivo imaging method to assess lymphatic draining function in the K/B×N mouse model of inflammatory arthritis. Indocyanine green (ICG), a near-infrared (NIR) fluorescent dye, was injected intradermally into the footpad of wild-type mice, the limb was illuminated with an 806 nm NIR laser, and the movement of ICG from the injection site to the draining popliteal lymph node (PLN) was recorded with a CCD camera. ICG-NIR images were analyzed to obtain 5 measures of lymphatic function across time. K/B×N arthritic mice and control non-arthritic littermates were imaged at one-month of age when acute joint inflammation commenced, and repeated at 3 months when joint inflammation became chronic. Lymphangiogenesis in PLNs was assessed by immunochemistry. ICG and its transport within lymphatic vessels were readily visualized and quantitative measures derived. During the acute phase of arthritis, the lymphatic vessels were dilated with increased ICG signal intensity and lymphatic pulses, and PLNs became fluorescent quickly. During the chronic phase, new lymphatic vessels were present near the foot. However, ICG appearance in lymphatic vessels was delayed. The size and area of PLN lymphatic sinuses progressively increased in the K/B×N mice. ICG-NIR lymphatic imaging is a valuable method to assess the lymphatic draining function in mice with inflammatory arthritis. ICG-NIR imaging of K/B×N mice identified two distinct lymphatic phenotypes during the acute and chronic phase of inflammation. This technique can be used to assess new therapies for lymphatic disorders.
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在慢性炎性关节炎的小鼠模型中，通过血管内皮生长因子受体3封锁抑制淋巴管生成和淋巴引流会增加炎症的严重程度。
影响因子：
--
作者：
Guo, Ruolin;Zhou, Quan;Proulx, Steven T.;Wood, Ronald;Ji, Rui-Cheng;Ritchlin, Christopher T.;Pytowski, Bronislaw;Zhu, Zhenping;Wang, Yong-Jun;Schwarz, Edward M.;Xing, Lianping
通讯作者：
Xing, Lianping
影响因子：
82.9
作者：
Tammela, Tuomas;Saaristo, Anne;Alitalo, Kari
通讯作者：
Alitalo, Kari
DOI：
10.1152/ajpheart.01223.2006
发表时间：
2007-06-01
影响因子：
4.8
作者：
Sharma, Ruchi;Wang, Wei;Sevick-Muraca, Eva M.
通讯作者：
Sevick-Muraca, Eva M.
DOI：
10.1152/ajpheart.1994.267.4.h1507
发表时间：
1994-10-01
影响因子：
4.8
作者：
LEU, AJ;BERK, DA;JAIN, RK
通讯作者：
JAIN, RK
影响因子：
4.8
作者：
Kobayashi, H;Kawamoto, S;Choyke, PL
通讯作者：
Choyke, PL