Toward intelligent synthetic neural circuits: directing and accelerating neuron cell growth by self-rolled-up silicon nitride microtube array.
Toward intelligent synthetic neural circuits: directing and accelerating neuron cell growth by self-rolled-up silicon nitride microtube array.
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DOI：
10.1021/nn504876y
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发表时间：
2014-11-25
期刊：
影响因子：
17.1
通讯作者：
Li, Xiuling
中科院分区：
文献类型：
--
作者：
Froeter, Paul;Huang, Yu;Cangellaris, Olivia V.;Huang, Wen;Dent, Erik W.;Gillette, Martha U.;Williams, Justin C.;Li, Xiuling
在神经接口平台中，培养通常在平坦、开放、刚性且不透明的基底上进行，这对反映大脑的原生微环境和与神经元的精确接合构成了挑战。在这里，我们提出了一个神经元细胞培养平台，由有序的微管阵列（直径2.7-4.4 μm），通过应变诱导自卷起纳米膜（s-RUM）技术形成的透明基板上的氮化硅（SiNx）薄膜（<40 nm）。这些微管表现出强大的物理限制和前所未有的指导作用，对原代皮层神经元的生长，与同轴限制配置类似的髓鞘。用连续活细胞成像评价微管内的动态神经生长，显示与微管外区域相比，微管内的生长速率显著增加（20倍）。我们属性的显着加速效果和精确的指导微管阵列的三维（3D）粘附和静电相互作用的氮化硅微管，分别。这项工作对通过安排微管阵列的大小、位置和模式来构建智能合成神经回路具有明确的意义，用于神经系统疾病的潜在治疗。
In neural interface platforms, cultures are often carried out on a flat, open, rigid, and opaque substrate, posing challenges to reflecting the native microenvironment of the brain and precise engagement with neurons. Here we present a neuron cell culturing platform that consists of arrays of ordered microtubes (2.7–4.4 μm in diameter), formed by strain-induced self-rolled-up nanomembrane (s-RUM) technology using ultrathin (<40 nm) silicon nitride (SiNx) film on transparent substrates. These microtubes demonstrated robust physical confinement and unprecedented guidance effect toward outgrowth of primary cortical neurons, with a coaxially confined configuration resembling that of myelin sheaths. The dynamic neural growth inside the microtube, evaluated with continuous live-cell imaging, showed a marked increase (20×) of the growth rate inside the microtube compared to regions outside the microtubes. We attribute the dramatic accelerating effect and precise guiding of the microtube array to three-dimensional (3D) adhesion and electrostatic interaction with the SiNx microtubes, respectively. This work has clear implications toward building intelligent synthetic neural circuits by arranging the size, site, and patterns of the microtube array, for potential treatment of neurological disorders.
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影响因子：
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作者：
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