Alterations of monoamine neurotransmitters, HPA-axis hormones, and inflammation cytokines in reserpine-induced hyperalgesia and depression comorbidity rat model.
Alterations of monoamine neurotransmitters, HPA-axis hormones, and inflammation cytokines in reserpine-induced hyperalgesia and depression comorbidity rat model.
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DOI：
10.1186/s12888-022-04065-0
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发表时间：
2022-06-22
期刊：
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
疼痛和抑郁常常同时发生，但这种情况的机制仍不清楚。本研究的目的是检查痛觉过敏和抑郁症合并症中单胺神经递质、下丘脑-垂体-肾上腺 (HPA) 轴激素和炎症细胞因子的变化。采用利血平诱导的“Sprague Dawley”（SD）大鼠模型，采用高效液相色谱仪检测中缝核区单胺类神经递质血清素（5-HT）、去甲肾上腺素（NE）、多巴胺（DA）及其代谢产物5-羟基吲哚乙酸（5-HIAA）、高香草酸（HVA）、3,4-二羟基苯乙酸（DOPAC）的浓度。色谱法（HPLC）。通过酶联免疫吸附测定法评估血清促肾上腺皮质激素（ACTH）、皮质醇（CORT）和炎症细胞因子白细胞介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α、干扰素（IFN）-γ、IL-4、IL-10的水平。重复注射利血平可诱发痛觉过敏和抑郁行为，并降低蔗糖偏好和水平运动距离，并增加强迫游泳试验中的不动时间。模型组中缝核5-HT、NE浓度升高，血清ACTH、CORT浓度升高。模型组血清IL-1β、IL-6升高，IL-10降低。需要在这些领域进行更多的研究来了解该疾病的发病机制，从而找到更多更好的治疗靶点。
Pain and depression often occur simultaneously, but the mechanism of this condition is still unclear. The aim of this study was to examine the alterations of monoamine neurotransmitters, hypothalamic–pituitary–adrenal (HPA) axis hormones, and inflammation cytokines in hyperalgesia and depression comorbidities. The reserpine-induced “Sprague Dawley” (SD) rat models were used, and the concentrations of monoamine neurotransmitters serotonin (5-HT), norepinephrine (NE), dopamine (DA), and their metabolic products 5-hydroxyindoleacetic acid (5-HIAA), Homovanillic acid (HVA), 3,4-Dihydroxyphenylacetic acid (DOPAC) in raphe nucleus region were tested by High Performance Liquid Chromatography (HPLC). Serum levels of Adrenocorticotropic Hormone (ACTH), Cortisol (CORT), and inflammatory cytokines interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, IL-4, IL-10 were assessed by enzyme linked immunosorbent assay. Repeated reserpine injection induced hyperalgesia and depressive behaviors with decreased sucrose preference and horizontal movement distance, and increased immobility time in forced swimming test. The concentrations of 5-HT and NE in raphe nucleus, and ACTH and CORT in serum were elevated in the model group. And the model group showed increases in serum IL-1β and IL-6, and decrease in serum IL-10. More research in these areas is needed to understand the pathogenesis of the disease, so as to find more and better therapeutic targets.
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DOI：
10.1126/science.1249240
发表时间：
2014-04-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Friedman AK;Walsh JJ;Juarez B;Ku SM;Chaudhury D;Wang J;Li X;Dietz DM;Pan N;Vialou VF;Neve RL;Yue Z;Han MH
通讯作者：
Han MH
影响因子：
2.7
作者：
Clark AK;Old EA;Malcangio M
通讯作者：
Malcangio M
影响因子：
10.6
作者：
Alba-Delgado, Cristina;Llorca-Torralba, Meritxell;Berrocoso, Esther
通讯作者：
Berrocoso, Esther
影响因子：
10.6
作者：
Dowlati, Yekta;Herrmann, Nathan;Lanctot, Krista L.
通讯作者：
Lanctot, Krista L.
影响因子：
5.3
作者：
Hale, Matthew W.;Dady, Kathleen F.;Lowry, Christopher A.
通讯作者：
Lowry, Christopher A.