An expanding role for purine uptake permease-like transporters in plant secondary metabolism.
An expanding role for purine uptake permease-like transporters in plant secondary metabolism.
复制标题
嘌呤摄取渗透酶样转运蛋白在植物继发代谢中的作用不断扩大。
DOI：
10.3389/fpls.2012.00078
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发表时间：
2012
影响因子：
5.6
通讯作者：
Jelesko JG
中科院分区：
文献类型：
--
作者：
Jelesko JG
在过去的十年中，我们的理解植物嘌呤摄取通透酶（PUP）转运蛋白家族主要是面向嘌呤核碱基底物和它们的组织特异性表达模式在拟南芥。然而，烟草PUP样同系物表现出尼古丁吸收渗透酶活性，最近被证明影响尼古丁代谢和根细胞生长。这些新发现扩展了PUP样转运蛋白的生理作用，包括植物次生代谢。PUP样转运蛋白的分子进化分析表明，它们是一个古老的药物和代谢物转运蛋白（DMT）超家族中的一个独特的类群。PUP类家族起源于陆生植物进化过程中，介于苔藓植物和石松植物之间。系统发育分析表明，PUP样转运蛋白可能来自DMT超家族中预先存在的核苷酸-糖转运蛋白家族。在石松属植物卷柏中，有三组旁系同源的PUP样转运蛋白。其中的三个PUP样旁系同源群体表现出广泛的基因复制和多样化的被子植物谱系内的模式，而更古老的PUP样旁系同源群体没有。四个密切相关的PUP样旁系同源物的生化特性与基于模型的系统发育分析表明被子植物PUP样转运蛋白的分子进化过程中的亚功能化和新功能化。这些发现表明，DMT转运蛋白的PUP样家族的成员可能参与不同的初级和次级植物代谢途径。
For the past decade, our understanding of the plant purine uptake permease (PUP) transporter family was primarily oriented on purine nucleobase substrates and their tissue-specific expression patterns in Arabidopsis. However, a tobacco PUP-like homolog demonstrating nicotine uptake permease activity was recently shown to affect both nicotine metabolism and root cell growth. These new findings expand the physiological role for PUP-like transporters to include plant secondary metabolism. Molecular evolution analyses of PUP-like transporters indicate they are distinct group within an ancient super family of drug and metabolite transporters (DMTs). The PUP-like family originated during terrestrial plant evolution sometime between the bryophytes and the lycophytes. A phylogenetic analysis indicates that the PUP-like transporters were likely derived from a pre-existing nucleotide-sugar transporter family within the DMT super family. Within the lycophyte Selaginella, there are three paralogous groups of PUP-like transporters. One of the three PUP-like paralogous groups showed an extensive pattern of gene duplication and diversification within the angiosperm lineage, whereas the more ancestral PUP-like paralogous groups did not. Biochemical characterization of four closely related PUP-like paralogs together with model-based phylogenetic analyses indicate both subfunctionalization and neofunctionalization during the molecular evolution of angiosperm PUP-like transporters. These findings suggest that members of the PUP-like family of DMT transporters are likely involved in diverse primary and secondary plant metabolic pathways.
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影响因子：
2.7
作者：
BALDWIN, IT;OHNMEISS, TE
通讯作者：
OHNMEISS, TE
影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
影响因子：
7.4
作者：
SOLT, ML
通讯作者：
SOLT, ML
影响因子：
11.6
作者：
Gillissen, B;Bürkle, L;Frommer, WB
通讯作者：
Frommer, WB
影响因子：
3
作者：
Dawson, RF
通讯作者：
Dawson, RF