Collagen type I of rat cortical and trabecular bone differs in the extent of posttranslational modifications
Collagen type I of rat cortical and trabecular bone differs in the extent of posttranslational modifications
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大鼠皮质骨和小梁骨的 I 型胶原蛋白翻译后修饰程度不同
DOI：
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发表时间：
2006
影响因子：
4.2
通讯作者：
L. Moro
中科院分区：
文献类型：
--
作者：
K. N. Suarez;M. Romanello;P. Bettica;L. Moro
本研究旨在评价皮质骨和松质骨基质的结构是否完全相同。为此，我们分析了I型胶原蛋白的一些翻译后修饰的程度，I型胶原蛋白是骨基质的主要成分。处死10只雌性和10只雄性100日龄大鼠，测定皮质骨和松质骨胶原的羟赖氨酸、糖基化羟赖氨酸和吡啶交联的含量。每种化合物的量表示为与羟脯氨酸的摩尔比。两种性别的胶原蛋白翻译后修饰模式似乎相同，但女性的差异程度高于男性。比较皮质骨和松质骨，前者含有较高量的羟基赖氨酸残基，而在后者中，羟基赖氨酸的糖基化较高，吡啶鎓交联浓度较低。此外，在雌性大鼠（r=-0.455，P =0.04）和雄性大鼠（r =-0.426;P=0.06）中，糖基化羟基赖氨酸和吡啶鎓交联浓度之间均建立了反向线性关系。本文讨论了这些发现可能意味着在功能方面。
This study sought to evaluate whether the architecture of the matrix of cortical and trabecular bone is exactly the same. For this purpose we analyzed the extent of some posttranslational modifications of type I collagen, which is the major component of bone matrix. Ten female and 10 male 100-day-old rats were sacrificed and the content of hydroxylysine, glycosylated hydroxylysine, and pyridinium cross-links of collagen from cortical and trabecular bone was determined. The amount of each compound was expressed as a molar ratio with hydroxyproline. The collagen posttranslational modification pattern appears to be the same in both sexes but with a higher extent of differences in females compared with males. Comparing cortical and trabecular bone, the former contains a higher amount of hydroxylysine residues whereas in the latter, glycosylation of hydroxylysine is higher and pyridinium cross-link concentration is lower. Moreover, an inverse linear relationship between glycosylated hydroxylysine and pyridinium crosslinks concentration was established, both for female (r=−0.455,P=0.04) and male rats (r=−0.426;P=0.06). This paper discusses what these findings may mean in functional terms.
DOI：
10.1016/0169-6009(92)90809-r
发表时间：
1992-09-01
期刊：
BONE AND MINERAL
影响因子：
--
作者：
FROST, HM;JEE, WSS
通讯作者：
JEE, WSS
DOI：
10.1016/s0169-6009(08)80050-5
发表时间：
1993-11-01
期刊：
BONE AND MINERAL
影响因子：
--
作者：
KALU, DN;SALERNO, E;SALIH, MA
通讯作者：
SALIH, MA
影响因子：
4.1
作者：
EYRE, DR;DICKSON, IR;VANNESS, K
通讯作者：
VANNESS, K