Elucidation of functional markers from Aspergillus nidulans developmental regulator FlbB and their phylogenetic distribution.
Elucidation of functional markers from Aspergillus nidulans developmental regulator FlbB and their phylogenetic distribution.
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DOI：
10.1371/journal.pone.0017505
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发表时间：
2011-03-10
期刊：
影响因子：
3.7
通讯作者：
Ugalde U
中科院分区：
文献类型：
--
作者：
Cortese MS;Etxebeste O;Garzia A;Espeso EA;Ugalde U
构巢曲霉是一种丝状真菌，广泛用作生物技术和临床研究的模型。它也被用作研究基本真核发育过程的平台。以前的研究确定和部分特征的一组蛋白质控制细胞的转变，在这个子囊菌。在这些蛋白质中，bZip型转录因子FlbB是生殖、应激反应和细胞死亡的关键调节因子。我们的目标是通过各种生物信息学方法预测FlbB内的关键功能残基和基序，以便为未来实验室实验的设计提供信息，并进一步了解控制真菌发育的分子机制。从盘菌亚门的40个成员中组装FlbB直系同源物和其关键相互作用伙伴FlbE的数据集。在FlbB的三个结构域中的每一个中鉴定出独特的特征。的N-末端区域编码一个bZip转录因子结构域与一个新的含组氨酸的DNA结合基序，而二聚化决定簇表现出两个不同的配置文件，按类隔离。FlbB的C-末端区域显示出与AP-1家族的应激反应调节剂的高度相似性，但具有按类别和顺序分离的保守半胱氨酸的可变模式。基序保守性分析表明，9个FlbB直系同源物属于Eurotiales命令包含一个基序的中心区域，可以介导与FlbE的相互作用。这里确定的关键残基和基序为后续实验研究的设计提供了基础。此外，FlbB直系同源物中这些残基和基序的存在或不存在可以帮助解释真菌物种之间发育程序的差异，以及定义推定的互补基团，可以用于将已知的功能特征扩展到其他物种。
Aspergillus nidulans is a filamentous fungus widely used as a model for biotechnological and clinical research. It is also used as a platform for the study of basic eukaryotic developmental processes. Previous studies identified and partially characterized a set of proteins controlling cellular transformations in this ascomycete. Among these proteins, the bZip type transcription factor FlbB is a key regulator of reproduction, stress responses and cell-death. Our aim here was the prediction, through various bioinformatic methods, of key functional residues and motifs within FlbB in order to inform the design of future laboratory experiments and further the understanding of the molecular mechanisms that control fungal development. A dataset of FlbB orthologs and those of its key interaction partner FlbE was assembled from 40 members of the Pezizomycotina. Unique features were identified in each of the three structural domains of FlbB. The N-terminal region encoded a bZip transcription factor domain with a novel histidine-containing DNA binding motif while the dimerization determinants exhibited two distinct profiles that segregated by class. The C-terminal region of FlbB showed high similarity with the AP-1 family of stress response regulators but with variable patterns of conserved cysteines that segregated by class and order. Motif conservation analysis revealed that nine FlbB orthologs belonging to the Eurotiales order contained a motif in the central region that could mediate interaction with FlbE. The key residues and motifs identified here provide a basis for the design of follow-up experimental investigations. Additionally, the presence or absence of these residues and motifs among the FlbB orthologs could help explain the differences in the developmental programs among fungal species as well as define putative complementation groups that could serve to extend known functional characterizations to other species.
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