Imaginal discs--a new source of chromosomes for genome mapping of the yellow fever mosquito Aedes aegypti.
Imaginal discs--a new source of chromosomes for genome mapping of the yellow fever mosquito Aedes aegypti.
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DOI：
10.1371/journal.pntd.0001335
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发表时间：
2011-10
影响因子：
3.8
通讯作者：
Sharakhov IV
中科院分区：
文献类型：
--
作者：
Sharakhova MV;Timoshevskiy VA;Yang F;Demin SIu;Severson DW;Sharakhov IV
埃及伊蚊是登革热和黄热病病毒的主要全球传播媒介。Ae的排序埃及的基因组刺激了媒介生物学和昆虫基因组学的研究。然而，目前的基因组组装是高度片段化的，只有1.31%的基因组被分配给染色体。缺乏一个可靠的染色体来源的物理作图一直是一个主要的障碍，以改善基因组组装的Ae。埃及人。在这项研究中，我们证明了从4龄幼虫的成虫盘有丝分裂染色体的细胞遗传学研究的效用。埃及人。每个载玻片制备物上的大量有丝分裂、大尺寸和单个染色体的可重复带型简化了细胞遗传学程序。根据染色体的带型结构，我们为三个山羊草中的每一个都绘制了特征图。将10个BAC克隆和18S rDNA探针定位于染色体的精确位置。研究表明，4龄幼虫的胚盘是山羊草有丝分裂染色体的上级来源。埃及人。所提出的方法允许DNA探针的染色体位置的精确映射，并可用于获得高质量的黄热病蚊子的基因组组装。登革热是一种新出现的健康威胁，威胁到全世界多达一半的人口。目前没有疫苗或药物治疗。因此，疾病预防主要是基于努力控制其主要蚊媒Ae。埃及人。新的媒介控制策略，如用无病原体的转基因蚊子取代种群，依赖于对蚊子基因组组织的详细了解。然而，目前Ae.埃及人是高度片段化的，需要额外的染色体物理作图。由于缺乏可读的多线染色体，这种蚊子的基因组图谱极具挑战性。在这项研究中，我们发现并调查了一个新的染色体来源，可用于细胞遗传学分析的山羊草。埃及伊蚊4龄幼虫成虫盘的有丝分裂染色体。利用这些染色体的自然带型，我们开发了一种新的基于带的方法，用于将DNA探针物理映射到精确的染色体位置。这种方法在基因组作图中的进一步应用将极大地提高现有Ae基因组序列组装草图的实用性。埃及伊蚊，从而促进应用先进的基因组技术来调查和开发登革热传播的新的遗传控制策略。
The mosquito Aedes aegypti is the primary global vector for dengue and yellow fever viruses. Sequencing of the Ae. aegypti genome has stimulated research in vector biology and insect genomics. However, the current genome assembly is highly fragmented with only ∼31% of the genome being assigned to chromosomes. A lack of a reliable source of chromosomes for physical mapping has been a major impediment to improving the genome assembly of Ae. aegypti. In this study we demonstrate the utility of mitotic chromosomes from imaginal discs of 4th instar larva for cytogenetic studies of Ae. aegypti. High numbers of mitotic divisions on each slide preparation, large sizes, and reproducible banding patterns of the individual chromosomes simplify cytogenetic procedures. Based on the banding structure of the chromosomes, we have developed idiograms for each of the three Ae. aegypti chromosomes and placed 10 BAC clones and a 18S rDNA probe to precise chromosomal positions. The study identified imaginal discs of 4th instar larva as a superior source of mitotic chromosomes for Ae. aegypti. The proposed approach allows precise mapping of DNA probes to the chromosomal positions and can be utilized for obtaining a high-quality genome assembly of the yellow fever mosquito. Dengue fever is an emerging health threat to as much as half of the human population around the world. No vaccines or drug treatments are currently available. Thus, disease prevention is largely based on efforts to control its major mosquito vector Ae. aegypti. Novel vector control strategies, such as population replacement with pathogen-incompetent transgenic mosquitoes, rely on detailed knowledge of the genome organization for the mosquito. However, the current genome assembly of Ae. aegypti is highly fragmented and requires additional physical mapping onto chromosomes. The absence of readable polytene chromosomes makes genome mapping for this mosquito extremely challenging. In this study, we discovered and investigated a new source of chromosomes useful for the cytogenetic analysis in Ae. aegypti – mitotic chromosomes from imaginal discs of 4th instar larvae. Using natural banding patterns of these chromosomes, we developed a new band-based approach for physical mapping of DNA probes to the precise chromosomal positions. Further application of this approach for genome mapping will greatly enhance the utility of the existing draft genome sequence assembly for Ae. aegypti and thereby facilitate application of advanced genome technologies for investigating and developing novel genetic control strategies for dengue transmission.
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影响因子：
3.5
作者：
Furey, TS;Haussler, D
通讯作者：
Haussler, D
影响因子：
1.6
作者：
Corradini, N;Rossi, F;Dimitri, P
通讯作者：
Dimitri, P
影响因子：
1.7
作者：
Demin, S.;Pleskach, N.;Solovjeva, L.
通讯作者：
Solovjeva, L.
影响因子：
2.6
作者：
Jiménez, LV;Kang, B;Severson, DW
通讯作者：
Severson, DW
DOI：
10.1126/science.1191864
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Arensburger P;Megy K;Waterhouse RM;Abrudan J;Amedeo P;Antelo B;Bartholomay L;Bidwell S;Caler E;Camara F;Campbell CL;Campbell KS;Casola C;Castro MT;Chandramouliswaran I;Chapman SB;Christley S;Costas J;Eisenstadt E;Feschotte C;Fraser-Liggett C;Guigo R;Haas B;Hammond M;Hansson BS;Hemingway J;Hill SR;Howarth C;Ignell R;Kennedy RC;Kodira CD;Lobo NF;Mao C;Mayhew G;Michel K;Mori A;Liu N;Naveira H;Nene V;Nguyen N;Pearson MD;Pritham EJ;Puiu D;Qi Y;Ranson H;Ribeiro JM;Roberston HM;Severson DW;Shumway M;Stanke M;Strausberg RL;Sun C;Sutton G;Tu ZJ;Tubio JM;Unger MF;Vanlandingham DL;Vilella AJ;White O;White JR;Wondji CS;Wortman J;Zdobnov EM;Birren B;Christensen BM;Collins FH;Cornel A;Dimopoulos G;Hannick LI;Higgs S;Lanzaro GC;Lawson D;Lee NH;Muskavitch MA;Raikhel AS;Atkinson PW
通讯作者：
Atkinson PW