Beaver and Naked Mole Rat Genomes Reveal Common Paths to Longevity.
Beaver and Naked Mole Rat Genomes Reveal Common Paths to Longevity.
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DOI：
10.1016/j.celrep.2020.107949
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发表时间：
2020-07-28
期刊：
影响因子：
8.8
通讯作者：
Gladyshev, Vadim N.
中科院分区：
文献类型：
--
作者：
Zhou, Xuming;Dou, Qianhui;Fan, Guangyi;Zhang, Quanwei;Sanderford, Maxwell;Kaya, Alaattin;Johnson, Jeremy;Karlsson, Elinor K.;Tian, Xiao;Mikhalchenko, Aleksei;Kumar, Sudhir;Seluanov, Andrei;Zhang, Zhengdong D.;Gorbunova, Vera;Liu, Xin;Gladyshev, Vadim N.
长寿啮齿类动物已成为研究衰老的一个有吸引力的模型。为了了解长寿的进化途径，我们准备了两种最长寿的啮齿动物——加拿大海狸（Castor canadensis）和裸鼹鼠（Heterocephalus glaber）的染色体水平基因组组装，并利用体外接近结扎和染色体构象捕获数据进行了支撑，并进行了长读测序。我们的比较基因组分析显示，“致病”位点的氨基酸替换在啮齿类动物基因组中普遍存在，而在长寿啮齿类动物中，相同的替换与常见的适应性表型有关，例如，对DNA损伤和细胞应激的抵抗力增强。通过新建立的替代模型和似然比检验，我们发现两种长寿啮齿动物的能量和脂肪酸代谢途径都富集了正选择信号。因此，高质量的长寿啮齿动物基因组资源有助于发现控制长寿和适应性进化的遗传因素。Zhou等人生成了加拿大海狸和裸鼹鼠的“染色体水平”基因组组装。他们描述了基因组特征，并确定了长寿啮齿动物中支持增强细胞对DNA损伤耐受性的常见替代。该研究也为衰老研究提供了宝贵的基因组资源。
Long-lived rodents have become an attractive model for the studies on aging. To understand evolutionary paths to long life, we prepare chromosome-level genome assemblies of the two longest-lived rodents, Canadian beaver (Castor canadensis) and naked mole rat (NMR, Heterocephalus glaber), which were scaffolded with in vitro proximity ligation and chromosome conformation capture data and complemented with long-read sequencing. Our comparative genomic analyses reveal that amino acid substitutions at “disease-causing” sites are widespread in the rodent genomes and that identical substitutions in long-lived rodents are associated with common adaptive phenotypes, e.g., enhanced resistance to DNA damage and cellular stress. By employing a newly developed substitution model and likelihood ratio test, we find that energy and fatty acid metabolism pathways are enriched for signals of positive selection in both long-lived rodents. Thus, the high-quality genome resource of long-lived rodents can assist in the discovery of genetic factors that control longevity and adaptive evolution. Zhou et al. generate “chromosome-level” genome assemblies for the Canadian beaver and naked mole rat. They characterize genome features and identify common substitutions in long-lived rodents that support enhanced tolerance of cells to DNA damage. The study also provides a valuable genome resource for aging research.
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影响因子：
8.8
作者：
Fang X;Seim I;Huang Z;Gerashchenko MV;Xiong Z;Turanov AA;Zhu Y;Lobanov AV;Fan D;Yim SH;Yao X;Ma S;Yang L;Lee SG;Kim EB;Bronson RT;Šumbera R;Buffenstein R;Zhou X;Krogh A;Park TJ;Zhang G;Wang J;Gladyshev VN
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作者：
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通讯作者：
Hartung F
影响因子：
5.3
作者：
Austad, SN
通讯作者：
Austad, SN
影响因子：
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作者：
Gao, LZ;Zhang, JZ
通讯作者：
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