The effects of age on the response of rabbit periosteal osteoprogenitor cells to exogenous transforming growth factor-beta 2.
The effects of age on the response of rabbit periosteal osteoprogenitor cells to exogenous transforming growth factor-beta 2.
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年龄对外源性转化生长因子-β2兔骨膜骨祖细胞反应的影响。
DOI：
--
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发表时间：
1994
影响因子：
4
通讯作者：
D. E. Ashhurst
中科院分区：
文献类型：
--
作者：
M. Critchlow;Y. Bland;D. E. Ashhurst
如果将转化生长因子-β注射到头盖骨或长骨的骨膜中，就会形成额外的骨和软骨。为了进一步研究这一点，将转化生长因子-β2注射到3日龄、3月龄和2岁兔的胫骨骨膜内。在所有病例中，骨膜形成层细胞(即骨祖细胞)的增殖增加，纤维层破裂。周围结缔组织出现水肿。在实验期间，正常新生儿的胫骨正在快速生长，有骨膜骨形成和骨内吸收。在实验性新生胫骨中，注射转化生长因子-β2 3次后骨膜成骨增加，5次注射后伴随有软骨形成；随着注射次数的增加，诱导骨和软骨的数量增加。4天后软骨细胞肥大，软骨被软骨内的骨取代。相比之下，在7次注射20 ng转化生长因子-β2后，3个月大的胫骨上只有少量的新骨，2岁的胫骨上没有新骨。7次注射200 ng转化生长因子-β2后1天，有少量的骨形成，7天后，3个月龄的胫骨上可见软骨形成小的离散结节，而2岁的胫骨上可见软骨内的小区域。结论：在本实验模型中，转化生长因子-β2的主要作用是促进骨膜内骨祖细胞的增殖。有观点认为，转化生长因子-β2不会启动成骨细胞或软骨细胞向成骨细胞或软骨细胞分化。提示它们的分化受局部环境，特别是血管和局部循环生长因子的控制。
Additional bone and cartilage are formed if transforming growth factor-beta is injected into the periosteum of calvariae or long bones. To investigate this further, transforming growth factor-beta 2 was injected into the periosteum of the tibia of 3-day-old, 3-month-old and 2-year-old rabbits. In all instances, there was an increase in proliferation of the cells of the cambial layer of the periosteum, that is, the osteoprogenitor cells, and breakdown of the fibrous layer. Oedema was induced in the surrounding connective tissues. Over the experimental period the normal neonatal tibia is undergoing rapid growth; there is periosteal bone formation and endosteal resorption. In the experimental neonatal tibiae, an increase in periosteal bone formation is seen after three injections of 20 ng of transforming growth factor-beta 2, which is accompanied by cartilage after five injections; the amounts of induced bone and cartilage increase with the number of injections. The chondrocytes hypertrophy after 4 days and the cartilage is replaced by bone endochondrally. In contrast, after seven injections of 20 ng transforming growth factor-beta 2, there is only a small amount of new bone on the 3-month-old tibia and none on the 2-year-old tibia. One day after seven injections of 200 ng transforming growth factor-beta 2, there is a small amount of bone formation, while seven days after cartilage is found as small discrete nodules on the 3-month-old tibia, but as small areas within the bone on the 2-year-old tibia. It is concluded that the primary effect of transforming growth factor-beta 2 in this experimental model is to increase the proliferative rate of the osteoprogenitor cells in the periosteum. It is argued that transforming growth factor-beta 2 does not initiate osteoblastic or chondrocytic differentiation of osteoprogenitor cells. It is suggested that their differentiation is controlled by the local environment, in particular, the vascularity and locally circulating growth factors.
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转化生长因子-β对发育中的小鼠次级腭的间充质细胞产生糖胺聚糖的调节。
影响因子：
2.7
作者：
D'Angelo,M;Greene,RM
通讯作者：
Greene,RM
影响因子：
4.2
作者：
L. Bonewald;G. Mundy
通讯作者：
L. Bonewald;G. Mundy
影响因子：
--
作者：
TURNER, RT;SPELSBERG, TC
通讯作者：
SPELSBERG, TC
DOI：
--
发表时间：
1987
期刊：
The American journal of pathology
影响因子：
--
作者：
Sprugel,KH;McPherson,JM;Clowes,AW;Ross,R
通讯作者：
Ross,R
转化和非转化生长因子存在于由胎鼠颅盖调节的培养基中。
DOI：
10.1073/pnas.82.21.7335
发表时间：
1985
影响因子：
11.1
作者：
Centrella,M;Canalis,E
通讯作者：
Canalis,E