Distinctively Expressed Cytokines by Three Different Inflammation Cells and Their Interaction with Keratinocytes in Wound Healing
Distinctively Expressed Cytokines by Three Different Inflammation Cells and Their Interaction with Keratinocytes in Wound Healing
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三种不同炎症细胞独特表达的细胞因子及其在伤口愈合中与角质形成细胞的相互作用
DOI：
10.1007/s10753-017-0655-9
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发表时间：
2017-09
期刊：
影响因子：
5.1
通讯作者：
Wu Yaojiong
中科院分区：
文献类型：
--
作者：
Wang Jingwen;Wang Xusheng;Chen Haiyan;Liu Jianjun;Tredget Edward E.;Wu Yaojiong
炎症细胞在创面愈合过程中起着至关重要的作用，但要进一步了解促进这一过程的关键因素，还需要进一步的探索。在本研究中，我们对从皮肤伤口分离的三种类型的炎症细胞进行了比较微阵列数据分析，并专注于差异表达的分泌因子。基因本体论浓缩和受体分析表明，Ly6C+炎性巨噬细胞中11个分泌因子基因和中性粒细胞中27个分泌因子基因有较高的表达(≥=5倍)，这些因子都被认为在角质形成细胞激活中具有潜在的重要作用。京都百科全书基因和基因组途径丰富分析表明，肿瘤坏死因子和白介素17信号通路是由Ly6C+炎性巨噬细胞分泌细胞因子激活的，而转化生长因子-β、缺氧诱导因子-1、核因子-κB和Rap1信号通路和调节干细胞多能性的信号通路与中性粒细胞分泌细胞因子密切相关。以肿瘤坏死因子为例，通过免疫荧光、基因敲除小鼠和定量磷酸蛋白质组学验证了细胞因子的来源及其对角质形成细胞的影响。综上所述，本研究表明细胞因子在三种炎症细胞中有不同的表达，这有助于识别诱导角质形成细胞信号转导和伤口愈合的关键因素。
Inflammatory cells exert crucial influence on wound healing, while exploration is still desired to get further insight into the key factors that promote the process. In the present study, we performed comparative microarray data analysis of the three types of inflammatory cells isolated from skin wounds and focused on differentially expressed secreted factors. Gene Ontology enrichment and receptor analysis indicated that 11 genes of secreted factors in Ly6C+inflammatory macrophages and 27 genes of secreted factors in neutrophils exhibited higher (≥ 5-fold) expression, and all these factors were considered as candidates with potentially important role in keratinocyte activation. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that TNF and IL17 signaling pathways were activated by Ly6C+inflammatory macrophages secreted cytokines, while TGF-beta, HIF-1, NF-κB, and Rap 1 signaling pathways and pathways regulating pluripotency of stem cells were highly involved with neutrophils secreted cytokines. Taking TNF as an example, the source of the cytokine and its impact on keratinocytes were verified by immunofluorescence, gene knockout mice, and quantitative phosphoproteomics. Collectively, this study has indicated distinctively expressed cytokines in three inflammatory cells, which is helpful in identifying key factors in inducing keratinocyte signaling and in wound healing.
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DOI：
--
发表时间：
2004
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Banno;Alix Gazel;M. Blumenberg
通讯作者：
T. Banno;Alix Gazel;M. Blumenberg
影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
2.4
作者：
Huat TJ;Khan AA;Pati S;Mustafa Z;Abdullah JM;Jaafar H
通讯作者：
Jaafar H
影响因子：
--
作者：
I. Pastar;Olivera Stojadinović;M. Tomic-Canic
通讯作者：
I. Pastar;Olivera Stojadinović;M. Tomic-Canic
影响因子：
3.1
作者：
Cross, KJ;Mustoe, TA
通讯作者：
Mustoe, TA