Quantitative Analysis of Apisin, a Major Protein Unique to Royal Jelly.
Quantitative Analysis of Apisin, a Major Protein Unique to Royal Jelly.
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DOI：
10.1155/2016/5040528
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ichihara K
中科院分区：
文献类型：
--
作者：
Furusawa T;Arai Y;Kato K;Ichihara K
蜂毒素是皇家浆（RJ）特有的蛋白质，已知其组成RJ蛋白质的大部分，并且作为含有主要皇家浆蛋白1和蜂毒素的异源寡聚体存在。然而，关于蜂毒肽定量方法的报道很少。因此，我们尝试使用HPLC（一种广泛使用的分析技术）定量蜂毒肽，如下所述。采用等电沉淀法和分子排阻层析法对该蛋白进行纯化，经SDS-PAGE和LC-MS分析鉴定为蜂毒肽。将纯化的蜂毒肽冻干，然后用于生成校准曲线以定量RJ中的蜂毒肽。蜂毒肽含量相当恒定（即，3.93至4.67w/w%）。这项研究是第一个描述了一个简单的，标准化的方法，用HPLC定量蜂毒肽，并建议蜂毒肽可以作为一个基准，为今后的RJ质量评价。
Apisin, a protein that is unique to royal jelly (RJ), is known to compose the greater part of the RJ proteins and to exist as a heterooligomer containing major royal jelly protein 1 and apisimin. However, few reports on the methods for quantifying apisin have been published. Thus, we attempted to quantify apisin using HPLC, a widely used analytical technique, as described below. Isoelectric precipitation and size-exclusion chromatography were used to obtain the purified protein, which was identified as apisin by SDS-PAGE and LC-MS analyses. The purified apisin was lyophilized and then used to generate a calibration curve to quantify apisin in RJ. The apisin content was fairly constant (i.e., 3.93 to 4.67 w/w%) in natural RJ. This study is the first to describe a simple, standardized method for quantifying apisin using HPLC and suggests that apisin can be used as a benchmark for future evaluations of RJ quality.
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