Development and characterization of a chemically defined food for Drosophila.

Development and characterization of a chemically defined food for Drosophila.
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DOI:
10.1371/journal.pone.0067308
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发表时间:
2013
期刊:
影响因子:
3.7
通讯作者:
Micchelli CA
Micchelli CA
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Lee WC;Micchelli CA

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饮食可以影响从行为到细胞新陈代谢的一系列生物过程。然而,个人饮食成分的确切作用可能是一个很难通过实验分离出来的变量。化学定义的食物(CDF)允许系统地评价单个的宏量和微量营养素。此外,CDF便于对独立于不同实验室的数据进行直接比较。在这里,我们报告了果蝇CDF的发展和特征。我们证明CDF可以支持实验室菌株的长期培养,并证明该配方在发育、寿命和生殖研究中从热量密度需求中分离出大量营养素具有实用价值。
Diet can affect a spectrum of biological processes ranging from behavior to cellular metabolism. Yet, the precise role of an individual dietary constituent can be a difficult variable to isolate experimentally. A chemically defined food (CDF) permits the systematic evaluation of individual macro- and micronutrients. In addition, CDF facilitates the direct comparison of data obtained independently from different laboratories. Here, we report the development and characterization of a CDF for Drosophila. We show that CDF can support the long-term culture of laboratory strains and demonstrate that this formulation has utility in isolating macronutrient from caloric density requirements in studies of development, longevity and reproduction.
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