Identification, Characterization and Functional Analysis of C-Class Genes Associated with Double Flower Trait in Carnation (Dianthus caryphyllus L.)
Identification, Characterization and Functional Analysis of C-Class Genes Associated with Double Flower Trait in Carnation (Dianthus caryphyllus L.)
复制标题
康乃馨重花性状相关C类基因的鉴定、表征及功能分析
DOI：
10.3390/plants9010087
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发表时间：
2020-01
期刊：
影响因子：
4.5
通讯作者：
Fu Xiaopeng
中科院分区：
文献类型：
--
作者：
Wang Qijian;Dan Naizhen;Zhang Xiaoni;Lin Shengnan;Bao Manzhu;Fu Xiaopeng
花瓣多的花更有观赏价值。AGAMOUS（AG）基因是拟南芥C类基因的核心成员，在拟南芥重瓣花的形成和雄蕊、心皮的鉴定中起着重要作用。我们在香石竹基因组中搜索了C类基因，发现了两个AG直向同源基因（DcaAGa，DcaAGb）。系统进化分析表明，这两个基因与euAG亚支的亲缘关系较近。然后，我们对其他几种豆科植物（甜菜、菠菜、藜）的基因组进行了C类基因的搜索，发现它们的C类基因都属于euAG亚支。半定量PCR（sq-PCR）分析表明，DcaAG基因在单花型中的表达量高于双花型。实时荧光定量PCR（qRT-PCR）分析表明，DcaAG基因在单花型和双花型香石竹的花蕾中的表达量与根、茎、叶中的表达量存在显著差异，DcaAG基因在香石竹的雄蕊和心皮中特异表达。此外，其它花器官特征基因（分别对应于A类、B类和E类基因的AP 1和AP 2、PI和AP 3、SEP 1和SEP 3）在单花型和重瓣型康乃馨的花器官中的表达量没有显著差异，表明C类（DcaAG）基因可能在重瓣型康乃馨中起重要作用。在35 S：：DcaAGa和35 S：：DcaAGb转基因拟南芥植株中观察到花瓣丢失或减少、早花、角果缩短和种子不育。这些结果表明，DcaAG基因可能对康乃馨花瓣数有负向影响，并在雄蕊和心皮发育中具有特异功能。
Flowers with more petals are of more ornamental value. It is well known that AGAMOUS (AG) is the core member of the C-class gene which plays an essential role in double flower formation and identification of stamens and carpels in Arabidopsis thaliana. We searched C-class genes in the genome of the carnation, and found two AG orthologs (DcaAGa, DcaAGb). Phylogenetic analysis showed that the two genes were closely related to the euAG subclade. Then we searched the genomes of other Caryophyllales plants (Beta vulgaris, Spinacia oleracea, Chenopodium quinoa) for C-class genes, and found that their C-class genes all belonged to the euAG subclade. Semi-quantitative PCR (sq-PCR) analysis indicated that the expression of DcaAG genes in the single flower phenotype was higher than that in the double flower phenotype. Quantitative real-time RT-PCR (qRT-PCR) analysis showed that the expressions of DcaAG genes in the flower bud were significantly different from those in the root, stem, and leaf between the single and double flower phenotype carnations, and that DcaAG genes were specifically expressed in the stamen and carpel of carnation. Moreover, the expression of other floral organ identity genes (AP1 and AP2, PI and AP3, SEP1 and SEP3 corresponding to the A-, B-, and E-class of genes, respectively) showed no significant difference in all floral organs between the single and double flower phenotype carnations, suggesting that C-class (DcaAG) genes might play an important role in the double flower phenotype in carnation. Petal loss or decrease, precocious flowering, silique shortening, and seed sterility were observed in 35S::DcaAGa and 35S::DcaAGb transgenic Arabidopsis plants. All these results show that DcaAG genes might affect the petal number negatively and have a specific function in stamen and carpel development in carnation.
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影响因子：
3.7
作者：
Chou KC;Shen HB
通讯作者：
Shen HB
DOI：
10.1093/dnares/dst053
发表时间：
2014-06
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
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作者：
Yagi M;Kosugi S;Hirakawa H;Ohmiya A;Tanase K;Harada T;Kishimoto K;Nakayama M;Ichimura K;Onozaki T;Yamaguchi H;Sasaki N;Miyahara T;Nishizaki Y;Ozeki Y;Nakamura N;Suzuki T;Tanaka Y;Sato S;Shirasawa K;Isobe S;Miyamura Y;Watanabe A;Nakayama S;Kishida Y;Kohara M;Tabata S
通讯作者：
Tabata S
影响因子：
3.3
作者：
A. Litt;V. Irish
通讯作者：
A. Litt;V. Irish
影响因子：
64.5
作者：
DREWS, GN;BOWMAN, JL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
影响因子：
4.3
作者：
A. Sharifi;K. Oizumi;S. Kubota;A. Bagheri;S. M. Shafaroudi;M. Nakano;A. Kanno
通讯作者：
A. Sharifi;K. Oizumi;S. Kubota;A. Bagheri;S. M. Shafaroudi;M. Nakano;A. Kanno