Bidirectional band-selective magnetization transfer along the protein backbone doubles the information content of solid-state NMR correlation experiments.
Bidirectional band-selective magnetization transfer along the protein backbone doubles the information content of solid-state NMR correlation experiments.
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DOI：
10.1007/s10858-017-0147-0
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发表时间：
2017-12
影响因子：
2.7
通讯作者：
Williamson PTF
中科院分区：
文献类型：
--
作者：
Jolly MM;Jarvis JA;Carravetta M;Levitt MH;Williamson PTF
共振归属是解决蛋白质结构的第一步。这通常通过采用单独的帧间和帧内残差实验来实现。通过利用混合旋转和旋转再耦合（MIRROR）条件，可以通过从CO到其相邻的Cα和随后的氨基酸的Cα的有效双向磁化转移来使信息含量加倍。我们已经将其纳入3D实验，3D-MIRROR-NCOCA，其中3D光谱中存在的相关性允许从单个实验中顺序分配蛋白质骨架，正如我们在GB 3的微晶制备中所证明的那样。此外，低功耗要求的镜子重新耦合序列，促进了低功耗的3D-NCOCA实验的发展。这使我们能够实现采集时间的显着减少，允许在不到24小时的时间内采集适合于GB 3的完整骨架共振分配的单个3D-NCOCA光谱。本文的在线版本（doi：10.1007/s10858-017-0147-0）包含补充材料，可供授权用户使用。
Resonance assignment is the first stage towards solving the structure of a protein. This is normally achieved by the employment of separate inter and intra residue experiments. By utilising the mixed rotation and rotary recoupling (MIRROR) condition it is possible to double the information content through the efficient bidirectional transfer of magnetization from the CO to its adjacent Cα and the Cα of the subsequent amino acid. We have incorporated this into a 3D experiment, a 3D-MIRROR-NCOCA, where correlations present in the 3D spectrum permit the sequential assignment of the protein backbone from a single experiment as we have demonstrated on a microcrystalline preparation of GB3. Furthermore, the low-power requirements of the MIRROR recoupling sequence facilitate the development of a low-power 3D-NCOCA experiment. This has enabled us to realise significant reductions in acquisition times, allowing the acquisition of a single 3D-NCOCA spectrum suitable for a full backbone resonance assignment of GB3 in less than 24 h. The online version of this article (doi:10.1007/s10858-017-0147-0) contains supplementary material, which is available to authorized users.
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影响因子：
2.7
作者：
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通讯作者：
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