An eQTL analysis of partial resistance to Puccinia hordei in barley.
An eQTL analysis of partial resistance to Puccinia hordei in barley.
复制标题
DOI：
10.1371/journal.pone.0008598
复制
发表时间：
2010-01-06
期刊：
影响因子：
3.7
通讯作者：
Waugh R
中科院分区：
文献类型：
--
作者：
Chen X;Hackett CA;Niks RE;Hedley PE;Booth C;Druka A;Marcel TC;Vels A;Bayer M;Milne I;Morris J;Ramsay L;Marshall D;Cardle L;Waugh R
对大麦锈菌引起的大麦叶锈病的遗传抗性涉及 R 基因和数量性状位点。 R 基因比数量性状基因座提供更高但更不持久的抗性。因此，探索定量或部分耐药性已成为控制疾病的有利选择。在双单倍体 Steptoe (St)/Morex (Mx) 作图群体中已鉴定出四个部分抗叶锈病的数量性状基因座。需要进一步研究来研究部分耐药性的分子机制并最终确定致病基因。我们使用遗传基因组学方法探索了对大麦叶锈病的部分抗性。我们在 St 和 Mx 幼苗以及 St/Mx 群体的 144 个双单倍体系中记录了 15K 安捷伦定制大麦微阵列上每个探针对应的 RNA 转录本丰度。总共有 1154 个和 1037 个基因在 St 和 Mx 中分别被鉴定为对大麦瘟疫菌有反应，并且在感染大麦瘟疫菌的 St 和 Mx 之间差异表达。使用来自 72 个远缘配对杂交的归一化比率，通过表达数量性状基因座 (eQTL) 作图，从 9557 个基因生成 15685 个 eQTL，来绘制转录本丰度变异的遗传决定因素。相关分析鉴定出128个与抗性相关的基因，其中89个eQTL与表型数量性状基因座（pQTL）共定位。亲本中的转录本丰度以及与水稻的同线性保守性使我们能够优先考虑 6 个基因作为 Rphq11（影响最大的 pQTL）的候选基因，并重点介绍其中一个，即磷脂氢过氧化物谷胱甘肽过氧化物酶 (HvPHGPx)，以进行详细分析。 eQTL 方法产生的信息导致鉴定出 pQTL 潜在的强候选基因，以抵抗大麦叶锈病和一般病原体反应途径。该数据集将有助于对宿主与病原体之间的相互作用进行系统评估，并可能有助于对该群体中测量的其他特征进行系统评估。
Genetic resistance to barley leaf rust caused by Puccinia hordei involves both R genes and quantitative trait loci. The R genes provide higher but less durable resistance than the quantitative trait loci. Consequently, exploring quantitative or partial resistance has become a favorable alternative for controlling disease. Four quantitative trait loci for partial resistance to leaf rust have been identified in the doubled haploid Steptoe (St)/Morex (Mx) mapping population. Further investigations are required to study the molecular mechanisms underpinning partial resistance and ultimately identify the causal genes. We explored partial resistance to barley leaf rust using a genetical genomics approach. We recorded RNA transcript abundance corresponding to each probe on a 15K Agilent custom barley microarray in seedlings from St and Mx and 144 doubled haploid lines of the St/Mx population. A total of 1154 and 1037 genes were, respectively, identified as being P. hordei-responsive among the St and Mx and differentially expressed between P. hordei-infected St and Mx. Normalized ratios from 72 distant-pair hybridisations were used to map the genetic determinants of variation in transcript abundance by expression quantitative trait locus (eQTL) mapping generating 15685 eQTL from 9557 genes. Correlation analysis identified 128 genes that were correlated with resistance, of which 89 had eQTL co-locating with the phenotypic quantitative trait loci (pQTL). Transcript abundance in the parents and conservation of synteny with rice allowed us to prioritise six genes as candidates for Rphq11, the pQTL of largest effect, and highlight one, a phospholipid hydroperoxide glutathione peroxidase (HvPHGPx) for detailed analysis. The eQTL approach yielded information that led to the identification of strong candidate genes underlying pQTL for resistance to leaf rust in barley and on the general pathogen response pathway. The dataset will facilitate a systems appraisal of this host-pathogen interaction and, potentially, for other traits measured in this population.
登录
查看更多内容
影响因子：
6.5
作者：
Arfaoui, A.;El Hadrami, A.;Cherif, M.
通讯作者：
Cherif, M.
影响因子：
3.3
作者：
Fu, JY;Jansen, RC
通讯作者：
Jansen, RC
影响因子：
1.8
作者：
Collinge, David B.;Jensen, Michael K.;Rung, Jesper
通讯作者：
Rung, Jesper
影响因子：
6.4
作者：
Berger, S;Papadopoulos, M;Roitsch, T
通讯作者：
Roitsch, T
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y