Species distribution and in vitro antifungal susceptibility of oral yeast isolates from Tanzanian HIV-infected patients with primary and recurrent oropharyngeal candidiasis.
Species distribution and in vitro antifungal susceptibility of oral yeast isolates from Tanzanian HIV-infected patients with primary and recurrent oropharyngeal candidiasis.
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DOI：
10.1186/1471-2180-8-135
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发表时间：
2008-08-12
期刊：
影响因子：
4.2
通讯作者：
Verweij PE
中科院分区：
文献类型：
--
作者：
Hamza OJ;Matee MI;Moshi MJ;Simon EN;Mugusi F;Mikx FH;Helderman WH;Rijs AJ;van der Ven AJ;Verweij PE
在坦桑尼亚，对HIV感染的原发性和复发性口咽念珠菌病患者的酵母菌分离株的物种分布和抗真菌药敏谱知之甚少。在坦桑尼亚达累斯萨拉姆的Muhimbili国家医院，从292名患有口咽念珠菌病的HIV感染患者中分离出了总共296株临床口腔酵母菌。使用标准表型方法进行酵母的鉴定。根据临床和实验室标准研究所（CLSI; M27-A2）的指南，使用肉汤微量稀释法评估了对氟康唑、伊曲康唑、咪康唑、克霉唑、阿替霉素B和制霉菌素的抗真菌药敏性。白色念珠菌是250例患者中最常见的菌种（84.5%），其次是念珠菌。glabrata 20例（6.8%）; 10例（3.4%）患者出现克柔氏病。原发性和复发性口咽念珠菌病患者之间的菌种分布没有观察到显著差异，但与从未接受过抗真菌治疗的患者相比，从既往接受过治疗的患者中培养的菌株对唑类化合物的敏感性显著降低。C.白色念珠菌是最常见的口咽念珠菌病患者分离的菌种。来自坦桑尼亚的口腔酵母菌株对测试的抗真菌剂具有高水平的敏感性。复发性口咽念珠菌病和既往抗真菌治疗与唑类抗真菌药物敏感性降低显著相关。
In Tanzania, little is known on the species distribution and antifungal susceptibility profiles of yeast isolates from HIV-infected patients with primary and recurrent oropharyngeal candidiasis. A total of 296 clinical oral yeasts were isolated from 292 HIV-infected patients with oropharyngeal candidiasis at the Muhimbili National Hospital, Dar es Salaam, Tanzania. Identification of the yeasts was performed using standard phenotypic methods. Antifungal susceptibility to fluconazole, itraconazole, miconazole, clotrimazole, amphotericin B and nystatin was assessed using a broth microdilution format according to the guidelines of the Clinical and Laboratory Standard Institute (CLSI; M27-A2). Candida albicans was the most frequently isolated species from 250 (84.5%) patients followed by C. glabrata from 20 (6.8%) patients, and C. krusei from 10 (3.4%) patients. There was no observed significant difference in species distribution between patients with primary and recurrent oropharyngeal candidiasis, but isolates cultured from patients previously treated were significantly less susceptible to the azole compounds compared to those cultured from antifungal naïve patients. C. albicans was the most frequently isolated species from patients with oropharyngeal candidiasis. Oral yeast isolates from Tanzania had high level susceptibility to the antifungal agents tested. Recurrent oropharyngeal candidiasis and previous antifungal therapy significantly correlated with reduced susceptibility to azoles antifungal agents.
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影响因子：
3.8
作者：
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